Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Talanta. 2017 Dec 1;175:121-126. doi: 10.1016/j.talanta.2017.07.024. Epub 2017 Jul 12.
A label-free nanopore biosensor for detection of DNA target is proposed utilizing hybridization chain reaction (HCR) strategy for signal amplification. The DNA target triggered HCR to form large DNA nanostructure inside the nanopore and out the nanopore membrane, which inducing the ionic current decrease effectively due to the blockage of the nanopore. The developed method achieves a desirable sensitivity of 30fM with a wide linear dynamic range from 0.1 to 10pM and demonstrated good application for real sample analysis. This work has great potential to be applied in the early diagnosis of gene-related diseases and provide a new paradigm for label-free nucleic acid amplification strategy in ultrasensitive nanopore biosensor.
提出了一种基于杂交链式反应（HCR）策略的无标记纳米孔生物传感器，用于检测 DNA 靶标。该传感器利用 DNA 靶标触发 HCR 在纳米孔内形成大的 DNA 纳米结构并穿出纳米孔膜，从而有效阻断纳米孔，导致离子电流降低。该方法具有良好的线性动态范围（0.1 到 10pM）和较高的灵敏度（30fM），并在实际样品分析中得到了很好的应用。这项工作在基因相关性疾病的早期诊断方面具有很大的应用潜力，并为超灵敏纳米孔生物传感器中的无标记核酸扩增策略提供了新的范例。
