Wang Xiaoqin, Stevens Kaden C, Ting Jeffrey M, Marras Alexander E, Rezvan Gelareh, Wei Xiaojun, Taheri-Qazvini Nader, Tirrell Matthew V, Liu Chang
Department of Chemical Engineering, University of South Carolina, Columbia, South Carolina 29208, USA.
Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA.
J Electrochem Soc. 2022 May;169(5). doi: 10.1149/1945-7111/ac6c55. Epub 2022 May 11.
DNAs have been used as probes for nanopore sensing of noncharged biomacromolecules due to its negative phosphate backbone. Inspired by this, we explored the potential of diblock synthetic polyelectrolytes as more flexible and inexpensive nanopore sensing probes by investigating translocation behaviors of PEO-b-PSS and PEO-b-PVBTMA through commonly used alpha-hemolysin (α-HL) and porin A (MspA) nanopores. Translocation recordings in different configurations of pore orientation and testing voltage indicated efficient PEO-b-PSS translocations through α-HL and PEO-b-PVBTMA translocations through MspA. This work provides insight into synthetic polyelectrolyte-based probes to expand probe selection and flexibility for nanopore sensing.
由于DNA的磷酸骨架带负电荷，因此已被用作纳米孔传感不带电生物大分子的探针。受此启发，我们通过研究聚环氧乙烷- b -聚苯乙烯磺酸钠（PEO-b-PSS）和聚环氧乙烷- b -聚（4-乙烯基苄基三甲基氯化铵）（PEO-b-PVBTMA）通过常用的α-溶血素（α-HL）和孔蛋白A（MspA）纳米孔的转位行为，探索了二嵌段合成聚电解质作为更灵活、更廉价的纳米孔传感探针的潜力。在不同孔取向和测试电压配置下的转位记录表明，PEO-b-PSS通过α-HL的转位以及PEO-b-PVBTMA通过MspA的转位是有效的。这项工作为基于合成聚电解质的探针提供了见解，以扩大纳米孔传感的探针选择和灵活性。
