应用辉光放电原子发射光谱法测定葡萄糖酸钙锌口服液及血样中钙和锌。
Determination of calcium and zinc in gluconates oral solution and blood samples by liquid cathode glow discharge-atomic emission spectrometry.
机构信息
Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
出版信息
Talanta. 2017 Dec 1;175:150-157. doi: 10.1016/j.talanta.2017.07.040. Epub 2017 Jul 14.
A novel flowing liquid cathode glow discharge (LCGD) was developed as an excitation source of the atomic emission spectrometry (AES) for the determination of Ca and Zn in digested calcium and zinc gluconates oral solution and blood samples, in which the glow discharge is produced between the electrolyte (as cathode) overflowing from a quartz capillary and the needle-like Pt anode. The electron temperature and electron density of LCGD were calculated at different discharge voltages. The discharge stability and parameters affecting the LCGD were investigated in detail. In addition, the measured results of real samples using LCGD-AES were verified by ICP-AES. The results showed that the optimized analytical conditions are pH = 1 HNO as supporting electrolyte, 4.5mLmin solution flow rate. The power consumption of LCGD is 43.5-66.0W. The R and the RSD ranged from 630 to 680V are 0.9942-0.9995 and 0.49%-2.43%, respectively. The limits of detections (LODs) for Zn and Ca are 0.014-0.033 and 0.011-0.097mgL, respectively, which are in good agreement with the closed-type electrolyte cathode atmospheric glow discharge (ELCAD). The obtained results of Ca and Zn in real samples by LCGD-AES are basically consistent with the ICP-AES and reference value. The results suggested that LCGD-AES can provide an alternative analytical method for the detection of metal elements in biological and medical samples.
一种新型流动液体阴极辉光放电（LCGD）被开发为原子发射光谱（AES）的激发源，用于测定消化后的葡萄糖酸钙和锌口服液和血液样本中的 Ca 和 Zn，其中辉光是在电解质（作为阴极）从石英毛细管中溢出并与针状 Pt 阳极之间产生的。在不同的放电电压下计算了 LCGD 的电子温度和电子密度。详细研究了 LCGD 的放电稳定性和影响放电的参数。此外，使用 LCGD-AES 对实际样品的测量结果通过 ICP-AES 进行了验证。结果表明，优化的分析条件为 pH=1 HNO3 作为支持电解质，4.5mLmin 溶液流速。LCGD 的功耗为 43.5-66.0W。R 和 RSD 范围从 630 到 680V 分别为 0.9942-0.9995 和 0.49%-2.43%。Zn 和 Ca 的检出限（LOD）分别为 0.014-0.033 和 0.011-0.097mgL，与封闭式电解质阴极大气压辉光放电（ELCAD）吻合良好。通过 LCGD-AES 获得的实际样品中 Ca 和 Zn 的结果与 ICP-AES 和参考值基本一致。结果表明，LCGD-AES 可为生物和医学样品中金属元素的检测提供替代分析方法。
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