College of Pharmaceutical Sciences, State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
College of Pharmaceutical Sciences, State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Talanta. 2017 Dec 1;175:217-223. doi: 10.1016/j.talanta.2017.07.035. Epub 2017 Jul 14.
By employing the attractive performance of fluorescent carbon dots and the assistant of hairpin structure, an innovative dual-channel biosensor on the basis of gold nanoparticles (AuNPs) for detecting multiple nucleotide sequences has been successfully proposed. In brief, the fluorescence of carbon dots (CDs) was quenched in the absence of the targets, and the hairpin structure was hybridized with the AuNPs-DNA and resulted in recovering the fluorescence. Instead, the presence of breast cancer (BRCA1) RNA/DNA could specifically bind with its contrary sequence to release the CDs from AuNPs, hence leading to the fluorescence recovery as a positive signal. Again, the hairpin structure can be released in the presence of thymidine kinase (TK1) RNA/DNA, thus induced a fluorescence quenching accordingly. Subsequently, the prepared sensing model was applied to detect BRCA1 RNA/DNA respectively accompanied with a linear range of 4-120nM as well as a detection limit of 1.5nM and 2.1nM, and 10-120nM as well as a detection limit of 3.6nM and 4.5nM for TK1 RNA/DNA respectively. More importantly, this sensing model could assay any possible gene sequence or aptamer-substrate complexes by appropriately programming.
采用荧光碳点的诱人性能和发夹结构的辅助，成功地提出了一种基于金纳米粒子（AuNPs）的创新双通道生物传感器，用于检测多种核苷酸序列。简而言之，在没有靶标的情况下，碳点（CDs）的荧光被猝灭，发夹结构与 AuNPs-DNA 杂交，导致荧光恢复。相反，乳腺癌（BRCA1）RNA/DNA 的存在可以特异性地与其相反的序列结合，从而将 CDs 从 AuNPs 上释放出来，从而导致荧光恢复作为阳性信号。此外，在胸苷激酶（TK1）RNA/DNA 的存在下，发夹结构可以被释放，从而相应地引起荧光猝灭。随后，将制备好的传感模型用于分别检测 BRCA1 RNA/DNA，其线性范围分别为 4-120nM 和检测限为 1.5nM 和 2.1nM，以及 10-120nM 和检测限为 3.6nM 和 4.5nM，用于 TK1 RNA/DNA。更重要的是，通过适当编程，该传感模型可以检测任何可能的基因序列或适体-底物复合物。
