Zanato Nicole, Talamini Lucas, Silva Tânia Regina, Vieira Iolanda Cruz
Department of Chemistry, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
Department of Chemistry, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
Talanta. 2017 Dec 1;175:38-45. doi: 10.1016/j.talanta.2017.07.021. Epub 2017 Jul 6.
This paper describes a simple, rapid and accurate label-free immunosensor for the determination of MC-LR in water samples. The anti-MC-LR was immobilized in a hybrid nanocomposite, comprised of exfoliated graphite nanoplatelets dispersed in a suspension of silver nanoparticles (AgNP-Nafion-xGnP), by adsorption. The nanomaterial obtained was then employed to prepare an immunosensor using a glassy carbon electrode (GCE) in one-step. Under optimized conditions, using square-wave voltammetry (SWV) and an incubation time of 10min, the immunosensor showed a linear relationship between the oxidation current and MC-LR concentration in the range of 0.5-5000ngmL with a detection limit of 0.017ngmL. The immunosensor demonstrated good precision, with coefficients of variation of 4.5% (inter-day; n = 7) and 3.1% (intra-day; n = 5), and acceptable selectivity (low interference in the presence of potential interferents), indicating that it is suitable for analytical applications. The immunosensor was successfully applied in the determination of MC-LR in a water sample and this platform offers advantages when compared to other electrochemical sensors reported in the literature, especially with regard to the time of incubation.
本文描述了一种用于测定水样中微囊藻毒素-LR（MC-LR）的简单、快速且准确的无标记免疫传感器。抗MC-LR通过吸附固定在一种由分散于银纳米颗粒（AgNP-Nafion-xGnP）悬浮液中的剥离石墨纳米片组成的混合纳米复合材料中。然后，将所得的纳米材料用于一步制备基于玻碳电极（GCE）的免疫传感器。在优化条件下，使用方波伏安法（SWV）且孵育时间为10分钟时，该免疫传感器在0.5 - 5000 ng/mL范围内的氧化电流与MC-LR浓度之间呈现线性关系，检测限为0.017 ng/mL。该免疫传感器具有良好的精密度，日间变异系数为4.5%（n = 7），日内变异系数为3.1%（n = 5），并且具有可接受的选择性（在存在潜在干扰物时干扰较低），表明其适用于分析应用。该免疫传感器成功应用于水样中MC-LR的测定，与文献中报道的其他电化学传感器相比，该平台具有优势，尤其是在孵育时间方面。
