De Lima A D, Singer W
J Comp Neurol. 1987 May 1;259(1):92-121. doi: 10.1002/cne.902590107.
The pontomesencephalic projection to the dorsal lateral geniculate nucleus (dLGN) of the cat was analyzed by combining retrograde transport of rhodamine-labeled latex spheres and immunohistochemistry. After injections of latex beads into the dLGN, sections of the brainstem were treated immunohistochemically for choline acetyltransferase (ChAT), serotonin (Ser), tyrosine hydroxylase (TH), and dopamine-beta-hydroxylase (DBH). Essentially, six regions in the brainstem contained retrogradely labeled cells: the superior colliculus, the parabigeminal nucleus, the dorsal raphe nuclei, the parabrachial area of the central tegmental field, the marginal nucleus of the brachium conjunctivum, and the nucleus coeruleus. Furthermore, isolated retrogradely labeled cells were present in the central nucleus of the raphe, in the cuneiform nucleus, and in the periaqueductal gray. Most serotoninergic double-labeled cells were found in the medial and lateral divisions of the dorsal raphe nuclei, but a few were also present in the central nucleus of the raphe. In the sections immunostained for ChAT, double-labeled cells were located in the central tegmental field, in the marginal nucleus of the brachium conjunctivum, and in the nucleus coeruleus. In the sections treated for TH and DBH, double-labeled cells showed a similar distribution, and like the ChAT(+) cells, they were located mainly in the central tegmental field, in the marginal nucleus of the brachium conjunctivum, and in the nucleus coeruleus. In these regions the cholinergic and noradrenergic cells that projected to the lateral geniculate nucleus were intermingled, the former predominating rostrally and the latter caudally. The majority of retrogradely labeled cells were located in the region of the central tegmental field in the vicinity of the brachium conjunctivum, and most of these cells were also ChAT-immunoreactive. We, therefore, conclude that the cholinergic projection is the most important of the central core projections ascending to the dLGN.
通过结合罗丹明标记乳胶微球的逆行运输和免疫组织化学方法，对猫脑桥中脑向背外侧膝状核（dLGN）的投射进行了分析。将乳胶珠注入dLGN后，对脑干切片进行胆碱乙酰转移酶（ChAT）、5-羟色胺（Ser）、酪氨酸羟化酶（TH）和多巴胺-β-羟化酶（DBH）的免疫组织化学处理。从本质上讲，脑干中有六个区域含有逆行标记细胞：上丘、副视束核、中缝背核、中央被盖区的臂旁区、结合臂边缘核和蓝斑核。此外，在中缝中央核、楔形核和导水管周围灰质中也存在孤立的逆行标记细胞。大多数5-羟色胺能双标记细胞见于中缝背核的内侧和外侧部，但在中缝中央核中也有少数。在ChAT免疫染色的切片中，双标记细胞位于中央被盖区、结合臂边缘核和蓝斑核。在TH和DBH处理的切片中，双标记细胞呈现类似的分布，并且与ChAT（+）细胞一样，它们主要位于中央被盖区、结合臂边缘核和蓝斑核。在这些区域，投射到外侧膝状核的胆碱能和去甲肾上腺素能细胞相互交织，前者在嘴侧占主导，后者在尾侧占主导。大多数逆行标记细胞位于结合臂附近的中央被盖区，并且这些细胞中的大多数也具有ChAT免疫反应性。因此，我们得出结论，胆碱能投射是向dLGN上行的中央核心投射中最重要的。
