Yamaleyeva Liliya M, Sun Yao, Bledsoe Tiffaney, Hoke Asia, Gurley Susan B, Brosnihan K Bridget
Department of Surgery/Hypertension and Vascular Research, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA;
Department of Radiology, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.
FASEB J. 2017 Dec;31(12):5520-5529. doi: 10.1096/fj.201700047RR. Epub 2017 Aug 21.
Accurate analysis of placental and fetal oxygenation is critical during pregnancy. Photoacoustic imaging (PAI) combines laser technology with ultrasound in real time. We tested the sensitivity and accuracy of PAI for analysis of placental and fetal oxygen saturation (sO) in mice. The placental labyrinth (L) had a higher sO than the junctional zone plus decidua region (JZ+D) in C57Bl/6 mice. Changing maternal O from 100 to 20% in C57Bl/6 mice lowered sO in these regions. C57Bl/6 mice were treated with the NO synthase inhibitor L-N-nitroarginine methyl ester (L-NAME) from gestational day (GD) 11 to GD18 to induce hypertension. L-NAME decreased sO in L and JZ+D at GD14 and GD18 in association with fetal growth restriction and higher blood pressure. Hypoxia-inducible factor 1α immunostaining was higher in L-NAME control mice at GD14. Fetal sO levels were similar between l-NAME and control mice at GD14 and GD18. In contrast to untreated C57Bl/6, L-NAME decreased placental sO at GD14 and GD18 GD10 or GD12. Placental sO was lower in fetal growth restriction in an angiotensin-converting enzyme 2 knockout mouse model characterized by placental hypoxia. On phantom studies, patterns of sO measured directly correlated with those measured by PAI. In summary, PAI enables the detection of placental and fetal oxygenation during normal and pathologic pregnancies in mice.-Yamaleyeva, L. M., Sun, Y., Bledsoe, T., Hoke, A., Gurley, S. B., Brosnihan, K. B. Photoacoustic imaging for quantification of placental oxygenation in mice.
孕期准确分析胎盘和胎儿的氧合情况至关重要。光声成像（PAI）实时将激光技术与超声技术相结合。我们测试了PAI分析小鼠胎盘和胎儿氧饱和度（sO）的敏感性和准确性。在C57Bl/6小鼠中，胎盘迷路（L）的sO高于交界区加蜕膜区（JZ+D）。将C57Bl/6小鼠的母体氧含量从100%降至20%会降低这些区域的sO。从妊娠第11天（GD）至第18天，用一氧化氮合酶抑制剂L-N-硝基精氨酸甲酯（L-NAME）处理C57Bl/6小鼠以诱导高血压。L-NAME在GD14和GD18时降低了L和JZ+D中的sO，同时伴有胎儿生长受限和血压升高。在GD14时，L-NAME处理组小鼠的缺氧诱导因子1α免疫染色更高。在GD14和GD18时，L-NAME处理组和对照组小鼠的胎儿sO水平相似。与未处理的C57Bl/6小鼠不同，L-NAME在GD14、GD18、GD10或GD12时降低了胎盘sO。在以胎盘缺氧为特征的血管紧张素转换酶2基因敲除小鼠模型中，胎儿生长受限情况下胎盘sO较低。在模型研究中，直接测量的sO模式与PAI测量的模式直接相关。总之，PAI能够检测正常和病理妊娠小鼠的胎盘和胎儿氧合情况。-亚马列耶娃，L.M.，孙，Y.，布莱索，T.，霍克，A.，格利，S.B.，布罗斯尼汉，K.B. 用于定量小鼠胎盘氧合的光声成像
