测量细菌染色体中的体内超螺旋动力学和转录延伸率。

Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes.

作者信息

Patrick Higgins N

机构信息

Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Bldg. 524A, 720 20th Street South, Birmingham, AL, 35233, USA.

出版信息

Methods Mol Biol. 2017;1624:17-27. doi: 10.1007/978-1-4939-7098-8_2.

DOI:10.1007/978-1-4939-7098-8_2

PMID:28842872

Abstract

DNA gyrase is the only topoisomerase that can catalytically introduce negative supercoils into covalently closed DNA. The enzyme plays a critical role in many phases of DNA biochemistry. There are only a few methods that allow one to measure supercoiling in chromosomal DNA and analyze the role of gyrase in transcription and its interaction with the other three bacterial topoisomerases. Here, we provide molecular tools for measuring supercoil density in the chromosome and for connecting the dots between transcription and DNA topology.

摘要

DNA促旋酶是唯一一种能够催化将负超螺旋引入共价闭合DNA的拓扑异构酶。该酶在DNA生物化学的许多阶段都起着关键作用。目前只有少数几种方法可用于测量染色体DNA中的超螺旋情况，并分析促旋酶在转录过程中的作用及其与其他三种细菌拓扑异构酶的相互作用。在此，我们提供了用于测量染色体中超螺旋密度以及连接转录与DNA拓扑结构之间关系的分子工具。