Visser Bryan J, Joshi Mohan C, Bates David
Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, TX, USA.
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Methods Mol Biol. 2017;1624:213-226. doi: 10.1007/978-1-4939-7098-8_16.
Fluorescence in situ hybridization (FISH) is a widely used technique to detect and localize specific DNA or RNA sequences in cells. Although supplanted in many ways by fluorescently labeled DNA binding proteins, FISH remains the only cytological method to examine many genetic loci at once (up to six), and can be performed in any cell type and genotype. These advantages have proved invaluable in studying the spatial relationships between chromosome regions and the dynamics of chromosome segregation in bacteria. A detailed protocol for DNA FISH in E. coli is described.
荧光原位杂交（FISH）是一种广泛应用于检测和定位细胞中特定DNA或RNA序列的技术。尽管在许多方面已被荧光标记的DNA结合蛋白所取代，但FISH仍然是唯一能同时检测多个（多达六个）基因位点的细胞学方法，并且可在任何细胞类型和基因型中进行。这些优势在研究细菌染色体区域之间的空间关系以及染色体分离动态方面已被证明具有极高的价值。本文描述了大肠杆菌中DNA FISH的详细实验方案。
