Kodaka Manami, Xu Xiaoyin, Yang Zeyu, Maruyama Junichi, Hata Yutaka
Department of Medical Biochemistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Department of Breast Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Methods Mol Biol. 2017;1668:127-134. doi: 10.1007/978-1-4939-7283-8_9.
Green fluorescent protein (GFP) is composed of 11 β-strands, and loses GFP signals, when divided into the N-terminal ten β-strands (GFP1-10) and the C-terminal last β-strand (GFP11). However, when GFP1-10 and GFP11 encounter, they reassemble into the fluorescent GFP. We expressed GFP1-10 and blasticidin resistance gene product-fused GFP11 (BSR-GFP11) in C2C12 cells. Both the cell lines do not show GFP but when they undergo myogenesis and myofusion, GFP1-10 and BSR-GFP11 form the fluorescent complex in multi-nuclear myotubes, so that GFP signals reflect myogenesis and myofusion.
绿色荧光蛋白（GFP）由11条β链组成，当被分为N端的十条β链（GFP1-10）和C端的最后一条β链（GFP11）时，会失去GFP信号。然而，当GFP1-10和GFP11相遇时，它们会重新组装成有荧光的GFP。我们在C2C12细胞中表达了GFP1-10和与杀稻瘟菌素抗性基因产物融合的GFP11（BSR-GFP11）。这两种细胞系均不显示GFP，但当它们进行肌生成和肌融合时，GFP1-10和BSR-GFP11在多核肌管中形成荧光复合物，从而使GFP信号反映肌生成和肌融合。
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