Department of Zoology, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India.
Department of Zoology, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India; Unit of Toxicology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.
Biomed Pharmacother. 2017 Nov;95:175-185. doi: 10.1016/j.biopha.2017.08.040. Epub 2017 Sep 12.
Berberine chloride (BC) has been widely used as an unprescribed oral drug in China for the treatment of various diseases, and it is an active ingredient in many traditionally used medicinal plants. The present study was undertaken to evaluate the beneficial effects of BC on oxidative stress, inflammation and apoptosis in the pancreas of Streptozotocin (STZ) induced diabetic rats. Diabetes was induced in the male Wistar albino rats using a single intraperitoneal injection of STZ of 40mg/kg b.w. Diabetic rats were treated with BC (50mg/kg b.w) for 45days. Oral administration of BC showed significant reduction of the blood glucose, glycated hemoglobin (HbAlc) and increased the plasma insulin, hemoglobin (Hb) and body weight. Lipid peroxidation marker LOOH and TBARS levels were reduced while SOD, CAT, GPx, GSH, vitamin E and vitamin C levels were increased in the pancreas. A significant reduction of pro-inflammatory mediators (TNF-α, NF-κB, phospho-NF-κB-p65, COX-2 and iNOS) and pro-apoptotic proteins (caspase-8, t-Bid, Bax, cytochrome-c and cleaved caspase-3), and elevation of anti-apoptotic protein Bcl-2, anti-inflammatory mediator IL-10 and GLUT-2 in were observed in BC treated diabetic rats. Thus, the present study demonstrated that BC has anti-hyperglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities in STZ induced diabetic rats.
盐酸小檗碱（BC）在中国被广泛用作未经处方的口服药物，用于治疗各种疾病，它是许多传统药用植物中的一种有效成分。本研究旨在评估 BC 对链脲佐菌素（STZ）诱导的糖尿病大鼠胰腺氧化应激、炎症和细胞凋亡的有益作用。雄性 Wistar 白化大鼠通过单次腹腔注射 40mg/kg b.w 的 STZ 诱导糖尿病。糖尿病大鼠用 BC（50mg/kg b.w）治疗 45 天。BC 的口服给药显示血糖、糖化血红蛋白（HbAlc）显著降低，血浆胰岛素、血红蛋白（Hb）和体重增加。胰腺中的脂质过氧化标志物 LOOH 和 TBARS 水平降低，而 SOD、CAT、GPx、GSH、维生素 E 和维生素 C 水平升高。在 BC 治疗的糖尿病大鼠中，观察到促炎介质（TNF-α、NF-κB、磷酸化-NF-κB-p65、COX-2 和 iNOS）和促凋亡蛋白（caspase-8、t-Bid、Bax、细胞色素-c 和 cleaved caspase-3）水平显著降低，抗凋亡蛋白 Bcl-2、抗炎介质 IL-10 和 GLUT-2 水平升高。因此，本研究表明 BC 对 STZ 诱导的糖尿病大鼠具有降血糖、抗氧化、抗炎和抗凋亡作用。
