Department of Zoology, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India.
Department of Zoology, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India.
Biomed Pharmacother. 2018 Mar;99:227-236. doi: 10.1016/j.biopha.2018.01.007.
The goal of the present study is to evaluate the effect of Berberine chloride (BC) on the liver of streptozotocin (STZ) induced diabetic rat. Diabetic rats were treated with BC (50 mg/kg b.w) or glibenclamide (6 mg/kg b.w), daily for 45 days. After BC treatment in diabetic rats, there was a significant (P < 0.05) decline in the levels of hepatic markers, lipid peroxidation markers such as lipid hydroperoxides (LOOH) and thiobarbituric acid reactive substances (TBARS), and pro-inflammatory mediators like tumor necrosis factor-alpha (TNF-α), phosphorylated nuclear factor kappa-B-p65 (phospho-NF-κB p65), cyclooxygenase (COX-2), nitric oxide synthase (iNOS) as well as pro-apoptotic mediators such as Bax and cytochrome c. A significant (P < 0.05) increase in hexokinase, glucose-6-phosphate dehydrogenase, enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), and non-enzymatic antioxidants such as glutathione (GSH), vitamin E and vitamin C, as well as anti-apoptotic protein Bcl-2 were observed in the liver of BC treated diabetic rats. Thus, from these findings, it can be concluded that the administration of BC notably recovered the liver from hyperglycemia induced antioxidant imbalance, inflammation and apoptosis as well as rectified the imbalance in carbohydrate metabolizing enzymes.
本研究旨在评估盐酸小檗碱（BC）对链脲佐菌素（STZ）诱导的糖尿病大鼠肝脏的影响。糖尿病大鼠每日用 BC（50mg/kg b.w）或格列本脲（6mg/kg b.w）处理 45 天。在糖尿病大鼠中用 BC 处理后，肝标志物、脂质过氧化标志物（如脂质氢过氧化物（LOOH）和硫代巴比妥酸反应物质（TBARS））、促炎介质（如肿瘤坏死因子-α（TNF-α））、磷酸化核因子κB-p65（磷酸化 NF-κB p65）、环氧化酶（COX-2）、一氧化氮合酶（iNOS）以及促凋亡介质（如 Bax 和细胞色素 c）的水平显著下降（P<0.05）。在 BC 处理的糖尿病大鼠肝脏中，己糖激酶、葡萄糖-6-磷酸脱氢酶、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）等酶抗氧化剂以及谷胱甘肽（GSH）、维生素 E 和维生素 C 等非酶抗氧化剂以及抗凋亡蛋白 Bcl-2 显著增加（P<0.05）。因此，从这些发现可以得出结论，BC 的给药显著恢复了肝脏的高血糖诱导的抗氧化失衡、炎症和细胞凋亡，并纠正了碳水化合物代谢酶的失衡。
