组胺及组胺受体拮抗剂对大鼠肠系膜微循环的影响。

Effect of histamine and histamine receptor antagonists on rat mesenteric microcirculation.

作者信息

Shahinian H K, Ferguson M K, Michelassi F

出版信息

J Surg Res. 1987 Jun;42(6):703-7. doi: 10.1016/0022-4804(87)90014-x.

Abstract

Histamine release adjacent to mesenteric arterioles under conditions of ischemia causes vasodilation and increases regional blood flow. This is presumably a protective mechanism which may be blocked by the use of H1 and H2 antagonists. Mesenteric arterioles of 29 rats were observed with intravital microscopy. Diameter and erythrocyte velocity were measured and arteriolar flow was calculated. Histamine was topically applied to the vessels under view in sequentially increasing concentrations (10(-7) to 10(-4) M). Following systemic injection of an H1 or H2 receptor antagonist or ibuprofen, the application of 10(-4) M histamine was repeated. Topical histamine caused vasodilation (122% of control; P less than 0.05) at 10(-4) M with a corresponding increase in erythrocyte velocity and calculated flow (118 and 177% of control, respectively; P less than 0.05 for each). The vasodilatory effects of histamine were blocked by systemic injection of histamine receptor antagonists (H1 + H2 greater than H1 greater than H2), while ibuprofen had no significant effect. In situations in which the gut is at risk for ischemia, the use of H1 and/or H2 receptor antagonists may seriously compromise the mesenteric microcirculation.

摘要

在缺血状态下，肠系膜小动脉附近组胺的释放会导致血管舒张并增加局部血流量。这可能是一种保护机制，而使用H1和H2拮抗剂可能会阻断该机制。通过活体显微镜观察29只大鼠的肠系膜小动脉。测量其直径和红细胞速度，并计算小动脉血流量。将组胺以浓度依次递增（10⁻⁷至10⁻⁴ M）的方式局部应用于观察的血管。在全身注射H1或H2受体拮抗剂或布洛芬后，重复应用10⁻⁴ M组胺。局部应用组胺在10⁻⁴ M时引起血管舒张（为对照的122%；P < 0.05），同时红细胞速度和计算出的血流量相应增加（分别为对照的118%和177%；每项P < 0.05）。组胺的血管舒张作用被组胺受体拮抗剂的全身注射所阻断（H1 + H2 > H1 > H2），而布洛芬没有显著影响。在肠道有缺血风险的情况下，使用H1和/或H2受体拮抗剂可能会严重损害肠系膜微循环。