CSIR-Central Leather Research Institute, Chennai 600 020, Tamil Nadu, India.
CSIR-Central Leather Research Institute, Chennai 600 020, Tamil Nadu, India.
Int J Biol Macromol. 2018 Jan;106:1032-1040. doi: 10.1016/j.ijbiomac.2017.08.111. Epub 2017 Aug 23.
Collagen is a unique protein abundantly present in the connective tissues of mammals and widely used for biomaterial preparation. In this study, we synthesized and characterized collagen-fucoidan blend films for tissue regenerative properties. Thermogravimetric analysis (TGA) and Differential Scanning Calorimetry (DSC) were used for thermal analysis of the blend films, and the films exhibited higher thermal stability and denaturation temperature (T) than those of native collagen due to intramolecular hydrogen bonding interaction between collagen and fucoidan, which was analyzed by FTIR spectroscopy. Morphological evaluation of these films using Scanning Electron Microscopy (SEM) showed smaller pore size than the control. Moreover, fucoidan protects collagen against enzymatic degradation and thereby increases the structural stability of collagen. Further, the in vitro studies of the synthesized films showed that they effectively facilitated the proliferation and migration of fibroblast cells without exhibiting toxicity. These study results suggested that the collagen-fucoidan blend films are a favorable substrate for growth of fibroblast cells, and may have great potential for tissue engineering applications.
胶原蛋白是一种存在于哺乳动物结缔组织中的独特蛋白质，广泛用于生物材料的制备。在这项研究中，我们合成并表征了胶原蛋白-褐藻聚糖共混膜，以研究其组织再生性能。通过热重分析（TGA）和差示扫描量热法（DSC）对共混膜进行了热分析，由于胶原蛋白和褐藻聚糖之间的分子内氢键相互作用，共混膜表现出比天然胶原蛋白更高的热稳定性和变性温度（T），这通过傅里叶变换红外光谱（FTIR）分析得到了证实。扫描电子显微镜（SEM）对这些膜的形貌评估表明，其孔径比对照样品更小。此外，褐藻聚糖可以保护胶原蛋白免受酶降解，从而提高胶原蛋白的结构稳定性。进一步的体外研究表明，这些合成膜能够有效地促进成纤维细胞的增殖和迁移，而没有表现出毒性。这些研究结果表明，胶原蛋白-褐藻聚糖共混膜是成纤维细胞生长的理想基质，可能在组织工程应用中有很大的潜力。
