Bernal-Ballen Andres, Lopez-Garcia Jorge-Andres, Ozaltin Kadir
Grupo de Investigación en Ingeniería Biomédica, Vicerrectoría de Investigaciones, Universidad Manuela Beltrán, Avenida Circunvalar No. 60-00, Bogotá 110231, Colombia.
Centre of Polymer Systems, Tomas Bata University in Zlín, Tr. Tomase Bati 5678, 76001 Zlín, Czech Republic.
Polymers (Basel). 2019 Aug 9;11(8):1325. doi: 10.3390/polym11081325.
Chitosan, fucoidan, and polyvinyl alcohol are categorized as polymers with biomedical applications. Ampicillin, on the other hand, is considered as an important antibiotic that has shown effectivity in both gram-positive and gram-negative micro-organisms. The aforementioned polymers possess unique properties that are considered desirable for cell regeneration although they exhibit drawbacks that can affect their final application. Therefore, films of these biomaterials were prepared and they were characterized using FTIR, SEM, XRD, degree of swelling and solubility, and MTT assay. The statistical significance of the experiments was determined using a two-way analysis of variance (ANOVA) with < 0.05. The characterization techniques demonstrated that the obtained material exhibits properties suitable for cell regeneration, and that a higher concentration of natural polymers promotes cells proliferation to a greater extent. The presence of PVA, on the other hand, is responsible for matrix stability and dictates the degree of swelling and solubility. The SEM images demonstrated that neither aggregations nor clusters were formed, which is favorable for the biological properties without detrimental to the morphological and physical features. Cell viability was comparatively similar in samples with and without antibiotic, and the physical and biological properties were not negatively affected. Indeed, the inherent bactericidal effect of chitosan was reinforced by the presence of ampicillin. The new material is an outstanding candidate for cell regeneration as a consequence of the synergic effect that each component provides to the blend.
壳聚糖、岩藻依聚糖和聚乙烯醇被归类为具有生物医学应用的聚合物。另一方面，氨苄青霉素被认为是一种重要的抗生素，对革兰氏阳性和革兰氏阴性微生物均显示出有效性。上述聚合物具有独特的性能，这些性能被认为对细胞再生是理想的，尽管它们存在一些可能影响其最终应用的缺点。因此，制备了这些生物材料的薄膜，并使用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、X射线衍射（XRD）、溶胀度和溶解度以及MTT法对其进行了表征。实验的统计学显著性通过双向方差分析（ANOVA）确定，显著性水平<0.05。表征技术表明，所获得的材料具有适合细胞再生的性能，并且较高浓度的天然聚合物能更大程度地促进细胞增殖。另一方面，聚乙烯醇的存在有助于基质稳定性，并决定溶胀度和溶解度。扫描电子显微镜图像表明，既没有形成聚集体也没有形成团簇，这有利于生物学性能，而不会对形态和物理特征产生不利影响。含抗生素和不含抗生素的样品中的细胞活力相对相似，并且物理和生物学性能没有受到负面影响。事实上，氨苄青霉素的存在增强了壳聚糖固有的杀菌效果。由于每种成分对共混物提供的协同效应，这种新材料是细胞再生的优秀候选材料。
