Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, Poland.
Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 15;189:436-442. doi: 10.1016/j.saa.2017.08.045. Epub 2017 Aug 14.
The effect of halothane, enflurane, sevoflurane, and isoflurane molecules, as volatile anesthetics, on the α-helices and polyproline II extended helices (PPII) of long-chain poly-l-lysine (PLL) were studied using Fourier-transform infrared and vibrational circular dichroism spectroscopy. Uncharged and charged α-helices, as well as charged extended PPII helices, were subjected to anesthetic actions in solvents with different pD values or methanol to water ratios. A crucial factor responsible for hindering the anesthetic-PLL interactions is shown to be the ionization of amino groups of the PLL side chains. The α-helix to β-sheet transition was triggered only for the uncharged α-helical structures of PLL by the nonpolar anesthetics under study.
采用傅里叶变换红外和振动圆二色光谱研究了卤烷、恩氟烷、七氟烷和异氟烷等挥发性麻醉剂分子对长链聚-L-赖氨酸（PLL）的α-螺旋和聚脯氨酸 II 延伸螺旋（PPII）的影响。非荷电和荷电α-螺旋以及荷电延伸 PPII 螺旋在不同 pD 值或甲醇与水比例的溶剂中受到麻醉剂作用。研究表明，侧链氨基的离子化是阻止麻醉剂与 PLL 相互作用的关键因素。只有研究中的非极性麻醉剂才能引发 PLL 的非荷电α-螺旋结构向β-折叠的转变。
