Trondsetås Lene, Mikkelsen Gustav, Lian Ingrid Alsos
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Clin Chem Lab Med. 2017 Nov 27;56(1):59-64. doi: 10.1515/cclm-2017-0263.
We recently observed that exposure to dry ice lowered sample pH and increased clotting times in lupus anticoagulant analyses, and that such changes could be prevented by placing samples at -80°C after dry ice exposure. In the current study, we sought to evaluate the effects of dry ice exposure on pH and various commonly used coagulation analyses.
Citrated plasma from 30 healthy blood donors was allocated to four preanalytical regimes: (1) immediate analysis of fresh plasma or (2) storage at -20°C; (3) storage at -20°C followed by dry ice exposure for 24 h or (4) storage at -20°C followed by dry ice exposure for 24 h and storage at -80°C for 24 h before analysis. Analyses of pH, prothrombin time international normalized ratio (PT-INR), activated partial thromboplastin time (APTT), antithrombin, fibrinogen, protein C and protein S was performed.
Samples exposed to dry ice had significantly lower pH, prolonged clotting times in PT-INR, APTT and fibrinogen analyses as well as lower levels of protein C, than samples not exposed to dry ice. These changes in coagulation analyses were not present if samples were stored at -80°C for 24 h after dry ice exposure. Antithrombin and protein S were not significantly affected by dry ice exposure.
Dry ice exposure lowered sample pH and affected various coagulation analyses. These effects were avoided by storing samples at -80°C for 24 h after dry ice exposure.
我们最近观察到，在狼疮抗凝物分析中，接触干冰会降低样本pH值并延长凝血时间，并且在接触干冰后将样本置于-80°C可防止此类变化。在本研究中，我们试图评估接触干冰对pH值和各种常用凝血分析的影响。
将来自30名健康献血者的枸橼酸盐血浆分配到四种分析前处理方案中：（1）新鲜血浆立即分析或（2）在-20°C储存；（3）在-20°C储存，随后接触干冰24小时，或（4）在-20°C储存，随后接触干冰24小时，并在分析前在-80°C储存24小时。进行pH值、凝血酶原时间国际标准化比值（PT-INR）、活化部分凝血活酶时间（APTT）、抗凝血酶、纤维蛋白原、蛋白C和蛋白S的分析。
与未接触干冰的样本相比，接触干冰的样本pH值显著降低，PT-INR、APTT和纤维蛋白原分析中的凝血时间延长，蛋白C水平降低。如果在接触干冰后将样本在-80°C储存24小时，则凝血分析中的这些变化不存在。抗凝血酶和蛋白S不受干冰暴露的显著影响。
接触干冰会降低样本pH值并影响各种凝血分析。在接触干冰后将样本在-80°C储存24小时可避免这些影响。
