一种快速的 LC-MS/MS 方法，用于测定玉米制品中的单端孢霉烯族毒素和这种真菌毒素的发生情况。
A rapid LC-MS/MS method for the determination of moniliformin and occurrence of this mycotoxin in maize products from the Bavarian market.
机构信息
Bavarian Health and Food Safety Authority, Veterinärstraße 2, 85764, Oberschleissheim, Germany.
Bavarian Health and Food Safety Authority, Eggenreuther Weg 43, 91058, Erlangen, Germany.
出版信息
Mycotoxin Res. 2018 Mar;34(1):9-13. doi: 10.1007/s12550-017-0293-y. Epub 2017 Aug 26.
A simple LC-MS/MS method was developed for the determination of moniliformin (MON) in maize and applied for the analysis of samples within the official food surveillance. The homogenized samples were extracted with acetonitrile/water 50/50 (v/v) which proved to have the highest extraction efficiency compared to other tested solvents. The centrifuged extracts were diluted with acetonitrile and were measured after chromatographic separation by HILIC (hydrophobic interaction liquid chromatography)-HPLC without any cleanup (dilute and shoot approach). The LOD and LOQ achieved by this procedure were 2.6 and 8.8 μg/kg, respectively. Thirty-nine samples of popcorn, maize meal, and semolina were collected in 2014 and 2015 at mills, cinemas, wholesale, and retail from the Bavarian market (Germany). The rate of contamination with MON was very high (97%) with levels ranging between the LOD and 847 μg/kg. The mean level was 118 μg/kg and the median, 39 μg/kg. The maximum value was detected in maize meal. The results are discussed with respect to possible health implications for the consumer.
建立了一种简单的 LC-MS/MS 方法，用于测定玉米中的单端孢霉烯族毒素（MON），并应用于官方食品监测中的样品分析。与其他测试溶剂相比，均质后的样品用乙腈/水 50/50（v/v）提取，显示出最高的提取效率。离心提取液用乙腈稀释，在不进行任何净化（稀释后直接进样）的情况下通过亲水作用色谱（HILIC）-HPLC 进行色谱分离后进行测量。该方法的检出限和定量限分别为 2.6 和 8.8μg/kg。2014 年和 2015 年，在巴伐利亚市场（德国）的磨坊、电影院、批发商和零售商处收集了 39 份爆米花、玉米粉和粗粒小麦粉样品。MON 污染率非常高（97%），含量范围在检出限和 847μg/kg 之间。平均水平为 118μg/kg，中位数为 39μg/kg。在玉米粉中检测到最高值。结果与消费者可能面临的健康影响有关进行了讨论。
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