Yu S R, Liu X J, Wang Y H, Liu J
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing, China.
Biomed Environ Sci. 1995 Dec;8(4):330-4.
Keshan Disease (KSD) is an endemic heart disease and moniliformin (MF) has been suggested as one of the etiological factors. In this paper, thin layer chromatographic (TLC) and high pressure liquid chromatographic (HPLC) methods were used for the determination of MF in the rice and corn samples collected from KSD areas and non-KSD areas. One hundred and twenty-three rice samples were analyzed and showed MF contamination in only 8 samples (KSD areas: 8.4% positive; non-KSD areas: 2.5% positive) ranging from 73.6 to 265.3 ng/g (mean concentration: KSD areas 156.3 ng/g; non-KSD areas 179.5 ng/g). One hundred and four corn samples in KSD areas and non-KSD areas were determinated by HPLC method, 45.2% samples were contaminated with MF (KSD areas: 81.4%; non-KSD areas: 19.7%) ranging from 52.3 to 1116.0 ng/g (mean concentration: KSD areas 488.9 ng/g; non-KSD areas 457.4 ng/g). The results showed that the contamination of MF in grains were significantly different between rice and corn, but not between the grains from the KSD areas and non-KSD areas, then casting doubt on the role of MF as an etiological factor of KS.
克山病（KSD）是一种地方性心脏病，有人认为串珠镰刀菌素（MF）是其病因之一。本文采用薄层色谱法（TLC）和高压液相色谱法（HPLC）测定了从克山病病区和非病区采集的水稻和玉米样品中的MF。分析了123份水稻样品，仅8份样品检测出MF污染（病区：阳性率8.4%；非病区：阳性率2.5%），污染范围为73.6至265.3 ng/g（平均浓度：病区156.3 ng/g；非病区179.5 ng/g）。采用HPLC法对病区和非病区的104份玉米样品进行了测定，45.2%的样品被MF污染（病区：81.4%；非病区：19.7%），污染范围为52.3至1116.0 ng/g（平均浓度：病区488.9 ng/g；非病区457.4 ng/g）。结果表明，谷物中MF的污染在水稻和玉米之间存在显著差异，但在病区和非病区的谷物之间没有显著差异，这对MF作为克山病病因的作用提出了质疑。
