de Oliveira F M, Carmona A M, Ladeira C
Escola Superior de Tecnologia da Saúde de Lisboa - Instituto Politécnico de Lisboa (ESTeSL/IPL), Av. D. João II, Lote 4.69.01, 1990-096 Lisbon, Portugal; The Institute of Cancer Research (ICR), 15 Cotswold Road, Sutton, SM2 5NG, Surrey, UK.
Escola Superior de Tecnologia da Saúde de Lisboa - Instituto Politécnico de Lisboa (ESTeSL/IPL), Av. D. João II, Lote 4.69.01, 1990-096 Lisbon, Portugal; School of Tourism and Maritime Technology, Polytechnic Institute of Leiria (ESTM/IPL). Campus 4, Santuário de Nossa Senhora dos Remédios, 2520-641 Peniche, Portugal.
Mutat Res Genet Toxicol Environ Mutagen. 2017 Oct;822:41-46. doi: 10.1016/j.mrgentox.2017.08.001. Epub 2017 Aug 5.
Electromagnetic fields (EMF) are classified as "possibly carcinogenic" by the International Agency for Research on Cancer (IARC). Some publications have reported associations between EMF exposure and DNA damage, but many other studies contradict such findings. Cytomorphological changes, such as micronuclei (MN), indicative of genomic damage, are biomarkers of genotoxicity. To test whether mobile phone-associated EMF exposure affects the MN frequency in exfoliated buccal cells, we obtained cells smears from the left and right inner cheeks of healthy mobile phone users, aged 18-30 (n=86), who also completed a characterization survey. MN frequencies were tested for potential confounding factors and for duration of phone use and preferential side of mobile phone use. No relationship was observed between MN frequency and duration of mobile phone use in daily calls. Cells ipsilateral to mobile phone use did not present a statistically significantly higher MN frequency, compared to cells contralateral to exposure. A highly statistically significant (p<0.0001) increase in MN frequency was found in subjects reporting regular exposure to genotoxic agents. Therefore, our results suggest that mobile phone-associated EMF do not to induce MN formation in buccal cells at the observed exposure levels.
电磁场（EMF）被国际癌症研究机构（IARC）归类为“可能致癌”。一些出版物报道了电磁场暴露与DNA损伤之间的关联，但许多其他研究与这些发现相矛盾。细胞形态学变化，如微核（MN），表明基因组损伤，是遗传毒性的生物标志物。为了测试与手机相关的电磁场暴露是否会影响脱落颊细胞中的微核频率，我们从18至30岁的健康手机用户（n = 86）的左右内侧脸颊获取细胞涂片，这些用户还完成了一项特征调查。对微核频率进行了潜在混杂因素、手机使用时长和手机使用偏好侧的测试。在日常通话中，未观察到微核频率与手机使用时长之间的关系。与暴露对侧的细胞相比，手机使用同侧的细胞微核频率并未呈现出统计学上的显著升高。在报告经常接触遗传毒性剂的受试者中，发现微核频率有高度统计学意义的增加（p < 0.0001）。因此，我们的结果表明，在所观察到的暴露水平下，与手机相关的电磁场不会诱导颊细胞中微核的形成。
