Kadeh Hamideh, Saravani Shirin, Moradi Mahsa, Alimanesh Niloofar
Oral and Dental Disease Research Center, Dept. of Oral & Maxillofacial Pathology, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.
Postgraduate Student, Dept. of Pediatric Dentistry, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.
J Dent (Shiraz). 2023 Mar;24(1 Suppl):118-124. doi: 10.30476/dentjods.2022.92515.1656.
Mobile usage has increased worldwide over the past two decades. There are conflicting reports about the carcinogenic effects of cell phone radiation on the oral mucosa. Micronucleus (MN) is considered a reliable marker for genotoxic damage.
This study aimed to identify the impact of mobile phone radiation on the MN frequency in oral mucosal cells.
In this descriptive-analytical study, 50 mobile phone users between the age group of 20-38 years were included. Samples were obtained from the right and left cheek mucosa of each subject (a total 100 cell samples). Every participant filled out a questionnaire about his or her cell phone usage habits. Additionally, personal information such as age, gender, and body mass index (BMI) were assessed. The Feulgen and Papanicolaou staining methods were used for staining of the cell samples. A total of 1000 cells in each sample were evaluated for MNs.
The mean number of MN in exposed and non-exposed mucosa by Feulgen method was 0.71±1.13 and 0.57±1.36, respectively. Also in Papanicolaou staining, the mean number of MN in the exposed mucosa and non-exposed mucosa was 6.94±6.61 and 6.54±6.88, respectively, but these differences were not significant (> 0.05). The frequency of MN in non-specific DNA staining was significantly (5- to 6-fold) higher than DNA-specific staining. We observed no statically significant differences between MN frequency according to age, gender, BMI, and other cell phone usage habits (> 0.05).
This study showed that cell phone use does not cause genotoxic effects in the buccal mucosa in the oral cavity. Moreover, using non-specific DNA staining methods can increase the frequency of MN by more than 5- to 6-fold.
在过去二十年中，全球范围内手机使用量有所增加。关于手机辐射对口腔黏膜致癌作用的报道相互矛盾。微核（MN）被认为是遗传毒性损伤的可靠标志物。
本研究旨在确定手机辐射对口腔黏膜细胞中微核频率的影响。
在这项描述性分析研究中，纳入了50名年龄在20 - 38岁之间的手机用户。从每个受试者的左右脸颊黏膜获取样本（共100个细胞样本）。每位参与者填写了一份关于其手机使用习惯的问卷。此外，还评估了年龄、性别和体重指数（BMI）等个人信息。细胞样本采用福尔根染色法和巴氏染色法进行染色。每个样本共评估1000个细胞的微核情况。
采用福尔根染色法时，暴露黏膜和未暴露黏膜中微核的平均数量分别为0.71±1.13和0.57±1.36。在巴氏染色中，暴露黏膜和未暴露黏膜中微核的平均数量分别为6.94±6.61和6.54±6.88，但这些差异不显著（>0.05）。非特异性DNA染色中微核频率显著高于DNA特异性染色（5至6倍）。根据年龄、性别、BMI和其他手机使用习惯，我们未观察到微核频率存在统计学显著差异（>0.05）。
本研究表明，使用手机不会对口腔颊黏膜产生遗传毒性作用。此外，使用非特异性DNA染色方法可使微核频率增加5至6倍以上。
