Zhu Hailiang, Wu Zhigang, Gadi Madhusudhan Reddy, Wang Shuaishuai, Guo Yuxi, Edmunds Garrett, Guan Wanyi, Fang Junqiang
National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Glycochemistry and Glycobiology, Shandong University, Jinan, Shandong 250100, China; Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA.
Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA.
Bioorg Med Chem Lett. 2017 Sep 15;27(18):4285-4287. doi: 10.1016/j.bmcl.2017.08.041. Epub 2017 Aug 19.
A cation exchange assisted binding-elution (BE) strategy for enzymatic synthesis of human milk oligosaccharides (HMOs) was developed. An amino linker was used to provide the cation ion under acidic condition which can be readily bound to cation exchange resin and then eluted off by saturated ammonium bicarbonate. Ammonium bicarbonate in the collections was easily removed by vacuum evaporation. This strategy circumvented the incompatible issue between glycosyltransferases and solid support or large polymers, and no purification was needed for intermediate products. With current approach, polyLacNAc backbones of HMOs and fucosylated HMOs were synthesized smoothly.
开发了一种用于酶促合成人乳寡糖（HMOs）的阳离子交换辅助结合-洗脱（BE）策略。使用氨基连接子在酸性条件下提供阳离子，该阳离子可容易地与阳离子交换树脂结合，然后用饱和碳酸氢铵洗脱。收集物中的碳酸氢铵通过真空蒸发很容易去除。该策略规避了糖基转移酶与固体支持物或大分子聚合物之间的不相容问题，中间产物无需纯化。采用当前方法，顺利合成了HMOs的聚乳糖胺骨架和岩藻糖基化HMOs。
