Department of Chemistry, Georgia State University, Atlanta, GA, 30303, USA.
Angew Chem Int Ed Engl. 2018 Dec 17;57(51):16638-16642. doi: 10.1002/anie.201810661. Epub 2018 Nov 26.
For decades, researchers have endeavored to develop a general automated system to synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and oligopeptides by commercially available machines. Inspired by the success of automated oligosaccharide synthesis through chemical glycosylation, a fully automated system is reported for oligosaccharides synthesis through enzymatic glycosylation in aqueous solution. The designed system is based on the use of a thermosensitive polymer and a commercially available peptide synthesizer. This study represents a proof-of-concept demonstration that the enzymatic synthesis of oligosaccharides can be achieved in an automated manner using a commercially available peptide synthesizer.
几十年来，研究人员一直致力于开发一种通用的自动化系统来合成寡糖，使其能够与商业可用的机器制备寡核苷酸和寡肽相媲美。受化学糖基化自动化寡糖合成成功的启发，本文报道了一种在水溶液中通过酶糖基化进行全自动化寡糖合成的系统。该设计的系统基于使用热敏聚合物和市售的肽合成仪。本研究证明了一个概念验证，即使用市售的肽合成仪可以以自动化的方式进行酶合成寡糖。
