Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
J Chin Med Assoc. 2018 Jan;81(1):25-30. doi: 10.1016/j.jcma.2017.05.009. Epub 2017 Aug 23.
Most polymorphisms that occur in TLR-2 are associated with gastrointestinal disorders such as peptic ulcer disease (PUD). Hence, in current study, association between TLR2-196 to -174 ins/del, Arg753Gln and Arg677Trp polymorphisms and risk of PUD development in north of Iran was evaluated.
This case-control study included 50 patients with PUD as cases and 50 people without peptic ulcer as control group. Blood and endoscopic biopsies were collected. Helicobacter pylori infection was screened by rapid urease test, specific IgG measurement and specific PCR for glmM gene. Then, TLR2-196 to -174 ins/del polymorphism was assessed by using allele-specific PCR. The Arg753Gln and Arg677Trp polymorphism in TLR2 gene were analyzed by the PCR-restriction fragment length polymorphism (RFLP).
There was no significant difference in the allele and genotype frequencies of polymorphisms in the TLR2-196 to -174 ins/ins and Arg753Gln genes between controls and patients, respectively. However, an association with increased risk for PUD was observed for polymorphism TLR-2 Arg677Trp (odds ratio [OR] = 7.9; 95% confidence interval [CI] = 0.94-67.5). Further analysis showed that H. pylori infection was associated with a significant difference in genotype and allele frequencies of TLR2-196 to -174 ins/ins and Arg753Gln polymorphism, respectively. Furthermore, there was no association between variant haplotypes and PUD development in H. pylori infected subjects. However, no association was detected between gender and genotypic frequencies of all polymorphisms in TLR2.
Our findings showed that TLR2 Arg677Trp polymorphism and H. pylori infection may play crucial roles in peptic ulcer development respectively in north of Iran.
TLR-2 中发生的大多数多态性与胃肠道疾病有关，如消化性溃疡病（PUD）。因此，在本研究中，评估了 TLR2-196 到-174 插入/缺失、Arg753Gln 和 Arg677Trp 多态性与伊朗北部 PUD 发病风险的关系。
本病例对照研究纳入 50 例 PUD 患者为病例组，50 例无消化性溃疡患者为对照组。采集血液和内镜活检。通过快速尿素酶试验、特异性 IgG 测定和 glmM 基因特异性 PCR 筛查幽门螺杆菌感染。然后，使用等位基因特异性 PCR 评估 TLR2-196 到-174 插入/缺失多态性。TLR2 基因的 Arg753Gln 和 Arg677Trp 多态性通过 PCR-限制性片段长度多态性（RFLP）分析。
在对照组和患者组中，TLR2-196 到-174 ins/ins 和 Arg753Gln 基因的多态性等位基因和基因型频率均无显著差异。然而，TLR-2 Arg677Trp 多态性与 PUD 发病风险增加相关（比值比[OR] = 7.9；95%置信区间[CI] = 0.94-67.5）。进一步分析显示，幽门螺杆菌感染与 TLR2-196 到-174 ins/ins 和 Arg753Gln 多态性的基因型和等位基因频率均存在显著差异。此外，在感染幽门螺杆菌的受试者中，变异单倍型与 PUD 发病无相关性。然而，在 TLR2 中，未发现性别与所有多态性的基因型频率之间存在相关性。
我们的研究结果表明，TLR2 Arg677Trp 多态性和幽门螺杆菌感染可能分别在伊朗北部的消化性溃疡发病中起关键作用。
