Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil; Graduate Program in Bio-experimentation, University of Passo Fundo, Passo Fundo, RS, Brazil.
J Ethnopharmacol. 2018 Jan 10;210:69-79. doi: 10.1016/j.jep.2017.08.033. Epub 2017 Aug 26.
Syzygium cumini (L.) Skeels is a plant widely used in folk medicine to treat diabetes mellitus (DM). The tea from its leaves is frequently used by diabetics for lowering hyperglycemia. There is a close relationship between DM and atherosclerosis, a chronic immuno-inflammatory disease, were the early stages encompass oxidative and glycative modifications in the structure of low density lipoprotein (LDL).
To investigate the potential protective effects of aqueous-leaf extract from Syzygium cumini (S.cExt) against CuSO-induced oxidation and methylglyoxal (MG)-induced glycation of human LDL in vitro.
LDL oxidative changes were evaluated by measuring conjugated dienes (CD) formation, thiobarbituric acid reactive substances (TBARS) levels, quenching of tryptophan (Trp) fluorescence and structural modifications in LDL particle. In LDL glycated by MG (glyLDL), we determined the levels of fluorescent advanced glycation end products (AGEs) and mobility by agarose gel electrophoresis.
S.cExt blocked oxidative events induced by CuSO in human LDL, plasma and serum. Fourier transform infrared spectroscopy (FT-IR) revealed that specific regions of apoB100 were oxidized by CuSO in human LDL and that S.cExt reduced these oxidations. Unlike, the increased AGEs levels and eletrophoretic mobility observed in LDL MG-glycated were not modified by S.cExt.
The findings herein indicate that S.cExt could be tested in atherogenesis models as potential protective agent against LDL oxidation.
Syzygium cumini（L.）Skeels 是一种在民间医学中广泛用于治疗糖尿病（DM）的植物。其叶子制成的茶经常被糖尿病患者用来降低高血糖。DM 与动脉粥样硬化密切相关，动脉粥样硬化是一种慢性免疫炎症性疾病，在早期阶段包含低密度脂蛋白（LDL）结构中的氧化和糖化修饰。
研究 Syzygium cumini（S.cExt）叶水提物对 CuSO 诱导的人 LDL 氧化和甲基乙二醛（MG）诱导的糖化的潜在保护作用。
通过测量共轭二烯（CD）形成、硫代巴比妥酸反应物质（TBARS）水平、色氨酸（Trp）荧光猝灭和 LDL 颗粒结构修饰来评估 LDL 氧化变化。在 MG 糖化的 LDL（glyLDL）中，我们测定了荧光晚期糖基化终产物（AGEs）的水平和琼脂糖凝胶电泳的迁移率。
S.cExt 阻止了 CuSO 诱导的人 LDL、血浆和血清中的氧化事件。傅里叶变换红外光谱（FT-IR）显示，CuSO 可氧化人 LDL 中的 apoB100 特定区域，而 S.cExt 可减少这些氧化。与在 LDL MG 糖化中观察到的增加的 AGEs 水平和电泳迁移率不同，S.cExt 不能修饰这些变化。
这些发现表明，S.cExt 可以在动脉粥样硬化模型中作为 LDL 氧化的潜在保护剂进行测试。
