Mahdavifard Sina, Nakhjavani Manochehr
Department of Clinical Biochemistry, Faculty of Medical Sciences, Ardabil University of Medical Sciences, Ardabil, Iran.
Endocrine Division, Vali-asr Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Med J Islam Repub Iran. 2020 May 13;34:47. doi: 10.34171/mjiri.34.47. eCollection 2020.
Thiamine deficiency contributes to hyperglycemia and diabetes complications. Thus, in this study, the effect of thiamine pyrophosphate (TPP) on the in vivo and in vitro formation of glycation, oxidative stress, and inflammatory markers (the main contributors of vascular diabetes complications) was examined in type 2 diabetes rat model. Type 2 diabetes was induced in rats with a combination of streptozotocin and nicotinamide (55+200 mg/kg). Two groups of rats, healthy and diabetic, were treated with 0.1% TPP in drinking water daily for 3 months and the 2 others received water only. The glucose, insulin, early to end glycation products, the activity of glyoxalase system, lipid profile, LDL oxidation markers, inflammatory markers, creatinine in the serum, and proteinuria in the urine of all rats were determined. Moreover, albumin and LDL were incubated with glucose in the presence and absence of TPP, and the samples were investigated for glycation and oxidation products. Different variables in all 4 groups were compared with multiple analysis of variance (MANOVA-Tukey) test using SPSS version 16. Significance level was set at p<0.05. TPP decreased the formation of diverse glycation and oxidation products in both in vivo (glycated LDL= 144.50±3.48 and oxidized LDL= 54.08±2.67 μmol/l) and in vitro (glycated LDL= 107.00±2.82 and oxidized LDL= 50.83±1.22 μmol/l). In addition, the vitamin reduced fasting blood sugar (9.23±0.29), insulin resistance (9.10±0.50), tumor necrosis factor-α (285.43±15.97), interleukin-6 (257.65±13.06), and improved the lipid profile, the activity of Glo system (Glo-I= 31.65±1.06 and Glo-II= 27.01±0.90 U/mL) and renal function in the diabetic rat (p<0.001). TPP decreased the major risk factors for diabetic complications and corrected the alternations of glucose and lipid metabolism in type 2 diabetic rats; thus, it is recommended for diabetes treatment.
硫胺素缺乏会导致高血糖和糖尿病并发症。因此，在本研究中，在2型糖尿病大鼠模型中检测了焦磷酸硫胺素（TPP）对体内和体外糖基化、氧化应激及炎症标志物（血管糖尿病并发症的主要促成因素）形成的影响。通过链脲佐菌素和烟酰胺（55 + 200 mg/kg）联合诱导大鼠患2型糖尿病。两组大鼠，健康组和糖尿病组，每天在饮用水中给予0.1% TPP，持续3个月，另外两组只给予水。测定了所有大鼠的血糖、胰岛素、早期至晚期糖基化产物、乙二醛酶系统活性、血脂、低密度脂蛋白氧化标志物、炎症标志物、血清肌酐和尿蛋白。此外，在有和没有TPP的情况下，将白蛋白和低密度脂蛋白与葡萄糖一起孵育，并对样品进行糖基化和氧化产物检测。使用SPSS 16版通过多变量方差分析（MANOVA - Tukey）检验比较所有4组中的不同变量。显著性水平设定为p < 0.05。TPP降低了体内（糖化低密度脂蛋白 = 144.50±3.48，氧化低密度脂蛋白 = 54.08±2.67 μmol/l）和体外（糖化低密度脂蛋白 = 107.00±2.82，氧化低密度脂蛋白 = 50.83±1.22 μmol/l）多种糖基化和氧化产物的形成。此外，该维生素降低了空腹血糖（9.23±0.29）、胰岛素抵抗（9.10±0.50）、肿瘤坏死因子-α（285.43±15.97）、白细胞介素-6（257.65±13.06），并改善了糖尿病大鼠的血脂、乙二醛酶系统活性（乙二醛酶-I = 31.65±1.06，乙二醛酶-II = 27.01±0.90 U/mL）和肾功能（p < 0.001）。TPP降低了糖尿病并发症的主要危险因素，并纠正了2型糖尿病大鼠葡萄糖和脂质代谢的异常；因此，推荐将其用于糖尿病治疗。
