Yin Sheng, Yan Yuzhang, Huang Teng, Guan Jingyuan, Wu Liying, Li Kun
Department of Oral and Maxillofacial Surgery, Xiangya School of Stomatology, Central South University, Changsha 410078; Department of Laboratory Medicine, Xiangya Medical School, Central South University, Changsha 410013, China.
Department of Oral and Maxillofacial Surgery, Xiangya School of Stomatology, Central South University, Changsha 410078, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2017 Jul 28;42(7):824-830. doi: 10.11817/j.issn.1672-7347.2017.07.014.
To determine bacteriostatic abilities of Artemisia argyi extracts, and to explore the effect of Artemisia argyi extracts on oral ulcer in rats. Methods: We extracted the mixture of Artemisia argyi volatile oils and water-extraction by leaching method and evaluated the anti-microbial effect of Artemisia argyi extracts on common oral floras in vitro. The rat cheeks were burnt by NaOH to establish the models of oral ulcer. The curative effects of crude drug of Artemisia argyi extracts at 2.0, 1.0, 0.5 g/mL on oral ulcer in rats were evaluated by measuring the oral ulcer healing time. Serum TNF-α level and expression of proliferating cell nuclear antigen (PCNA) were analyzed by ELASA and immunohistochemical staining. Results: Artemisia argyi extracts obviously inhibited the Staphylococcus aureus and Streptococcus. NaOH-made oral ulcer in rats were successfully established. The crude drug at 2.0 and 1.0 g/mL obviously reduced healing time, significantly inhibited the release of TNF-α, and improved the PCNA level in the ulcer tissues (All P<0.01). The extracts obviously reduced the local inflammatory reaction and promoted tissue repair of oral ulcer. Conclusion: Artemisia argyi extracts promote tissue repair of oral ulcer via inhibiting bacterial growth, reducing the release of TNF-α and improving the PCNA level.
探讨艾叶提取物的抑菌能力，以及其对大鼠口腔溃疡的影响。方法：采用浸渍法提取艾叶挥发油与水提取物的混合物，体外评价艾叶提取物对常见口腔菌群的抗菌作用。用氢氧化钠烧灼大鼠面颊建立口腔溃疡模型，通过测量口腔溃疡愈合时间评价艾叶提取物生药2.0、1.0、0.5 g/mL对大鼠口腔溃疡的疗效。采用酶联免疫吸附测定法（ELASA）和免疫组化染色法分析血清肿瘤坏死因子-α（TNF-α）水平和增殖细胞核抗原（PCNA）的表达。结果：艾叶提取物能明显抑制金黄色葡萄球菌和链球菌。成功建立了氢氧化钠致大鼠口腔溃疡模型。2.0和1.0 g/mL的生药明显缩短愈合时间，显著抑制TNF-α的释放，并提高溃疡组织中PCNA水平（均P<0.01）。提取物明显减轻口腔溃疡局部炎症反应，促进组织修复。结论：艾叶提取物通过抑制细菌生长、减少TNF-α释放和提高PCNA水平促进口腔溃疡组织修复。
