Miao Mingsan, Peng Mengfan, Xing Zetian, Liu Dandan
Department of Pharmacology, Henan University of Chinese Medicine, Zhengzhou, Henan, China.
Henan Tai Long Pharmaceutical Limited by Share Ltd, Zhengzhou, Henan, China.
Saudi J Biol Sci. 2019 May;26(4):790-794. doi: 10.1016/j.sjbs.2019.02.005. Epub 2019 Feb 16.
To observe the effect of common clinical drug Shuangjin Lian mixture on rats with oral ulcer and discuss its mechanism.
Sodium carboxymethyl cellulose caused leukocyte aggregation in rats, observed the anti-inflammatory effect of Shuangjin mixture. 3 mm * 3 mm size ulcer surface in the oral cavity of rats was caused by 90% phenol solution, to observe the therapeutic effect and anti-inflammatory effect of Shuangjinlian mixture on rats with oral ulcers.
The low, middle and high dose Shuangjinlian mixture can inhibits the accumulation of white blood cells caused by sodium carboxymethyl cellulose in rats significantly ( < 0.01). And reduce the degree of edema and hyperaemia around the ulcer tissue significantly ( < 0.01), improve the ulcer healing probability, and reduce the level of TNF-α, VEGF levels and increase IL-2 level in the serum of rats with oral ulcers significantly ( < 0.01). Pathological examination showed that the lesion of ulcer tissue in each treatment group was obviously alleviated.
Shuangjinlian mixture had anti-inflammatory effect and was effective for the prevention and treatment of oral ulcer in rats.
观察临床常用药物双黄连合剂对口腔溃疡大鼠的影响并探讨其作用机制。
用羧甲基纤维素钠致大鼠白细胞聚集，观察双黄连合剂的抗炎作用。用90%苯酚溶液造成大鼠口腔内3 mm * 3 mm大小的溃疡面，观察双黄连合剂对口腔溃疡大鼠的治疗作用及抗炎作用。
低、中、高剂量双黄连合剂均可显著抑制羧甲基纤维素钠所致大鼠白细胞的聚集（<0.01），并显著减轻溃疡组织周围的水肿和充血程度（<0.01），提高溃疡愈合几率，显著降低口腔溃疡大鼠血清中TNF-α、VEGF水平并升高IL-2水平（<0.01）。病理检查显示各治疗组溃疡组织病变明显减轻。
双黄连合剂具有抗炎作用，对大鼠口腔溃疡的防治有效。
