通过质谱法鉴定人胎盘中的强啡肽1-8。

Identification of dynorphin 1-8 in human placenta by mass spectrometry.

作者信息

Ahmed M S, Randall L W, Sibai B, Dass C, Fridland G, Desiderio D M, Tolun E

出版信息

Life Sci. 1987 May 25;40(21):2067-76. doi: 10.1016/0024-3205(87)90099-3.

Abstract

The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M + H) ion in the fast atom bombardment mode. This octa-peptide could be the natural ligand of the kappa opioid receptors present in the human placental villus tissue.

摘要

人胎盘绒毛组织含有阿片受体和肽。从绒毛组织中提取的阿片肽通过反相高效液相色谱法和放射受体测定法进行分离。强啡肽1-8的存在通过快速原子轰击模式下（M + H）离子的质谱产生得到证实。这种八肽可能是人胎盘绒毛组织中存在的κ阿片受体的天然配体。