Yuan Yuan, Zhou Jun-Hui, Huang Lu-Qi
State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2016 Jan;41(1):139-143. doi: 10.4268/cjcmm20160127.
The stress effect is a characteristic of Dao-di herbs caused by environmental gene expression of medical plants is influenced by environmental changes and finally affects the formation and accumulation of metabolites. Using Scutellaria baicalensis as material, active component of wild type of S. baicalensis from 19 production areas were analysed; It was found that climate change can influence the accumulation of active components. Then, S. baicalensis suspension cells was exposed to various environments, and enzyme activity and gene expression were measured, indicating the molecular mechanism of stress effect on S. baicalensis. Hence, we found the prerequisite and method to study the stress effect on Dao-di herbs, and we hope this research can provides some references for another studies of Dao-di herbs.
胁迫效应是道地药材的一个特性,药用植物的环境基因表达受环境变化影响,最终影响代谢产物的形成与积累。以黄芩为材料,分析了来自19个产地的野生型黄芩的活性成分;发现气候变化会影响活性成分的积累。然后,将黄芩悬浮细胞置于不同环境中,测定酶活性和基因表达,揭示胁迫对黄芩作用的分子机制。因此,我们找到了研究道地药材胁迫效应的前提条件和方法,希望本研究能为道地药材的其他研究提供一些参考。