Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Göttingen, Germany.
J Am Chem Soc. 2017 Sep 13;139(36):12378-12381. doi: 10.1021/jacs.7b06412. Epub 2017 Aug 30.
We designed cell-permeant red-emitting fluorescent dye labels with >140 nm Stokes shifts based on 9-iminoanthrone, 9-imino-10-silaxanthone, and 9-imino-10-germaxanthone fluorophores. The corresponding probes selectively targeting mitochondria, lysosomes, and F-actin demonstrate low toxicity and enable stimulated emission depletion (STED) nanoscopy in neurons, human fibroblasts, U2OS, and HeLa cells. In combination with known small Stokes shift dyes, our probes allow live-cell three-color STED nanoscopy of endogenous targets on popular setups with 775 nm STED wavelength.
我们设计了基于 9-亚氨基蒽酮、9-亚氨基-10-硅蒽酮和 9-亚氨基-10-锗蒽酮荧光团的具有>140nm 斯托克斯位移的细胞通透性红色荧光染料标签。相应的探针选择性地靶向线粒体、溶酶体和 F-肌动蛋白，表现出低毒性，并能够在神经元、人成纤维细胞、U2OS 和 HeLa 细胞中进行受激发射损耗（STED）纳米显微镜术。与已知的小斯托克斯位移染料结合，我们的探针允许在具有 775nm STED 波长的流行设置上对活细胞内源性靶标进行三色 STED 纳米显微镜术。
