Natural Constituents Research Center, Korea Institute of Science and Technology (KIST) , Gangneung, Gangwon-do 25451, Republic of Korea.
Natural Products Research Institute, College of Pharmacy, Seoul National University , Seoul 151-742, Republic of Korea.
J Nat Prod. 2017 Sep 22;80(9):2542-2546. doi: 10.1021/acs.jnatprod.7b00293. Epub 2017 Aug 28.
Despite numerous efforts to discover novel bioactive products from microorganisms, previously reported compounds are repetitively reisolated. A new polyketide glycoside, gordonic acid (1), isolated from the mixed culture of two Gram-positive bacteria, Gordonia sp. KMC005 and Streptomyces tendae KMC006, is reported. The structure of 1 was characterized as an acyclic polyene polyketide substituted with a β-d-digitoxopyranose through NMR, HR-ESI-QTOF-MS, IR, and UV spectral data. The stereochemistry for 1 was determined by Mosher's method followed by 2D NOESY analysis and by NMR chemical shift calculations supported by DP4 analysis. Gordonic acid (1) showed weak activity against Micrococcus luteus and Enterococcus hirae.
尽管人们已经做出了许多努力来从微生物中发现新的生物活性产物，但以前报道的化合物仍在不断被重新分离出来。从革兰氏阳性菌 Gordonia sp. KMC005 和 Streptomyces tendae KMC006 的混合培养物中分离到了一种新的聚酮糖苷，即 Gordonic 酸（1）。通过 NMR、HR-ESI-QTOF-MS、IR 和 UV 光谱数据，确定了 1 的结构为带有β-D-地芰糖的无环多烯聚酮。通过 Mosher 法、二维 NOESY 分析以及 DP4 分析支持的 NMR 化学位移计算确定了 1 的立体化学。Gordonic 酸（1）对微球菌和肠球菌表现出较弱的活性。
