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诱导放线菌基因组中隐秘的次生代谢物基因簇激活。

Elicitation for activation of the actinomycete genome's cryptic secondary metabolite gene clusters.

作者信息

El-Hawary Seham S, Hassan Marwa H A, Hudhud Ahmed O, Abdelmohsen Usama Ramadan, Mohammed Rabab

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, Cairo University Cairo Egypt.

Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University Beni-Suef 62511 Egypt

出版信息

RSC Adv. 2023 Feb 16;13(9):5778-5795. doi: 10.1039/d2ra08222e. eCollection 2023 Feb 14.

DOI:10.1039/d2ra08222e
PMID:36816076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9932869/
Abstract

This review summarizes the recent advances in the elicitation approaches used to activate the actinomycete genome's cryptic secondary metabolite gene clusters and shows the diversity of natural products obtained by various elicitation methods up to June 2022, such as co-cultivation of actinomycetes with actinomycetes, other non-actinomycete bacteria, fungi, cell-derived components, and/or algae. Chemical elicitation and molecular elicitation as transcription factor decoys, engineering regulatory genes, the promoter replacement strategy, global regulatory genes, and reporter-guided mutant selection were also reported. For researchers interested in this field, this review serves as a valuable resource for the latest studies and references.

摘要

本综述总结了用于激活放线菌基因组中隐秘次级代谢产物基因簇的诱导方法的最新进展，并展示了截至2022年6月通过各种诱导方法获得的天然产物的多样性，例如放线菌与放线菌、其他非放线菌细菌、真菌、细胞衍生成分和/或藻类的共培养。还报道了化学诱导和作为转录因子诱饵的分子诱导、工程调控基因、启动子替换策略、全局调控基因以及报告基因引导的突变体选择。对于该领域感兴趣的研究人员来说，本综述是最新研究和参考文献的宝贵资源。

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