Avoli M, Olivier A
Neurosci Lett. 1987 May 6;76(2):249-54. doi: 10.1016/0304-3940(87)90724-5.
Intracellular recordings were performed in human neocortical neurons in 'in vitro' slices of brain samples excised during surgical treatment of epilepsy. In 14 of 38 neurons obtained from cortex exhibiting interictal spiking, bursts of action potentials arising from a synaptic depolarizing potential could be elicited by extracellular focal stimulation of adequate strength. The N-methyl-D-aspartate (NMDA) antagonist 2-amino-5-phosphonovalerate (APV) was capable of reducing and eventually blocking these bursts without affecting the repetitive firing evoked by depolarizing intracellular pulses or the membrane input resistance. These data suggest a role played by NMDA receptors in the bursting activity displayed by human neurons from spiking cortical areas and demonstrate a potential use of NMDA antagonists as antiepileptic drugs.
在癫痫手术治疗期间切除的脑样本的“体外”切片中的人类新皮层神经元中进行了细胞内记录。在从表现出发作间期尖峰的皮层获得的38个神经元中的14个中，通过足够强度的细胞外局部刺激可以引发由突触去极化电位产生的动作电位爆发。N-甲基-D-天冬氨酸（NMDA）拮抗剂2-氨基-5-磷酸戊酸（APV）能够减少并最终阻断这些爆发，而不影响由去极化细胞内脉冲诱发的重复放电或膜输入电阻。这些数据表明NMDA受体在来自尖峰皮层区域的人类神经元所表现出的爆发活动中发挥的作用，并证明了NMDA拮抗剂作为抗癫痫药物的潜在用途。
