Niem Jennifer Millera, Billones-Baaijens Regina, Stodart Benjamin J, Reveglia Pierluigi, Savocchia Sandra
Gulbali Institute, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia.
Faculty of Science and Health, School of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia.
Plants (Basel). 2023 May 28;12(11):2132. doi: 10.3390/plants12112132.
Grapevine trunk diseases (GTDs) impact the sustainability of vineyards worldwide and management options are currently limited. Biological control agents (BCAs) may offer a viable alternative for disease control. With an aim to develop an effective biocontrol strategy against the GTD pathogen , this study investigated the following: (1) the efficacy of the strains in suppressing the BD pathogen in detached canes and potted vines; (2) the ability of a strain of (BCA17) to colonize and persist within grapevine tissues; and (3) the mode of action of BCA17 to antagonize . Co-inoculations of the antagonistic bacterial strains with revealed that one strain of (BCA17) suppressed infection by 100% and 80% in detached canes and potted vines, respectively. Stem inoculations of a laboratory-generated rifampicin-resistant strain of BCA17 in potted vines (cv. Shiraz) indicated the bacterial strain could colonize and persist in the grapevine tissues, potentially providing some protection against GTDs for up to 6 months. The bioactive diffusible compounds secreted by BCA17 significantly reduced the spore germination and fungal biomass of and the other representative GTD pathogens. Complementary analysis via MALDI-TOF revealed the presence of an unknown cyclic lipopeptide in the bioactive diffusible compounds, which was absent in a non-antagonistic strain of (JMN13), suggesting this novel lipopeptide may be responsible for the biocontrol activity of the BCA17. Our study provided evidence that BCA17 is a potential BCA to combat , with a potential novel mode of action.
葡萄树干病害（GTDs）影响着全球葡萄园的可持续发展，目前的管理方法有限。生物防治剂（BCAs）可能为病害防治提供一种可行的替代方案。为了制定一种有效的针对GTD病原体的生物防治策略，本研究调查了以下内容：（1）这些菌株在离体茎杆和盆栽葡萄藤中抑制BD病原体的效果；（2）一种菌株（BCA17）在葡萄组织中定殖和持续存在的能力；以及（3）BCA17拮抗的作用方式。将拮抗细菌菌株与[具体病菌名称未给出]共同接种表明，一种[具体细菌名称未给出]菌株（BCA17）在离体茎杆和盆栽葡萄藤中分别将感染抑制了100%和80%。在盆栽葡萄藤（设拉子品种）中对实验室产生的对利福平耐药的BCA17菌株进行茎部接种表明，该细菌菌株能够在葡萄组织中定殖并持续存在，可能为GTDs提供长达6个月的某种保护。BCA17分泌的生物活性可扩散化合物显著降低了[具体病菌名称未给出]以及其他代表性GTD病原体的孢子萌发和真菌生物量。通过基质辅助激光解吸电离飞行时间质谱（MALDI - TOF）进行的补充分析表明，生物活性可扩散化合物中存在一种未知的环脂肽，而在一种非拮抗的[具体细菌名称未给出]菌株（JMN13）中不存在，这表明这种新型脂肽可能是BCA17生物防治活性的原因。我们的研究提供了证据，表明BCA17是一种对抗[具体病菌名称未给出]的潜在生物防治剂，具有潜在的新作用方式。
