Aron D C, Hosta L P, Vargo E B, Andrews P C, Roos B A
Peptides. 1987 Mar-Apr;8(2):207-11. doi: 10.1016/0196-9781(87)90091-x.
We have identified a system, the TT human medullary thyroid carcinoma cell line, which we found to contain 31.3 +/- 27.7 ng of somatostatin 28(1-12) immunoreactivity/mg protein. Radioimmunoassay of gel filtration fractions showed that the major form of immunoreactive somatostatin 28(1-12) had a molecular weight of 1,500 daltons. During reversed-phase high pressure liquid chromatography, this 1,500-dalton species coeluted with synthetic somatostatin 28(1-12). Somatostatin 28(1-12) containing forms larger than 7,000 daltons were also observed. Further studies will be required to elucidate the route of processing of prosomatostatin. The fact that the products of prosomatostatin processing in these cells are similar to those in normal tissues indicates that the TT medullary thyroid carcinoma cell line constitutes a useful model for human somatostatin gene expression.
我们已经鉴定出一种体系，即TT人甲状腺髓样癌细胞系，我们发现其每毫克蛋白质中含有31.3±27.7纳克的生长抑素28(1 - 12)免疫反应性。凝胶过滤组分的放射免疫测定表明，免疫反应性生长抑素28(1 - 12)的主要形式分子量为1500道尔顿。在反相高效液相色谱过程中，这种1500道尔顿的物质与合成的生长抑素28(1 - 12)共洗脱。还观察到分子量大于7000道尔顿的含生长抑素28(1 - 12)的形式。需要进一步研究以阐明前生长抑素的加工途径。这些细胞中前生长抑素加工产物与正常组织中的产物相似，这一事实表明TT甲状腺髓样癌细胞系构成了人类生长抑素基因表达的有用模型。
