Firner Sara, Zaucke Frank, Michael Joern, Dargel Jens, Schiwy-Bochat Karl-Heinz, Heilig Juliane, Rothschild Markus Alexander, Eysel Peer, Brüggemann Gert-Peter, Niehoff Anja
Institute of Biomechanics and Orthopaedics, German Sport University Cologne, Cologne, Germany.
Dr. Rolf M. Schwiete Research Unit for Osteoarthritis, Orthopaedic University Hospital Friedrichsheim gGmbH, Frankfurt, Germany.
J Histochem Cytochem. 2017 Oct;65(10):593-606. doi: 10.1369/0022155417729154. Epub 2017 Aug 28.
Perifibrillar adapter proteins, interconnecting collagen fibrils, and linking the collagen network with the aggrecan matrix seem to play a crucial role in the pathogenesis of osteoarthritis (OA). Therefore, we examined immunohistochemically the extracellular distribution of collagen II and the main perifibrillar adapter proteins-collagen IX, decorin, cartilage oligomeric matrix protein (COMP), and matrilin-3-in human samples of healthy (n=4) and OA (n=42) knee joint cartilage. Histopathology assessment was performed using an OA score. Staining patterns were evaluated in relation to the disease stage. The perifibrillar adapter proteins were uniformly distributed in the upper zones of healthy cartilage. In moderate OA (n=8; score 14.3 ± 4.7), all proteins analyzed were locally absent in the fibrillated area or the superficial and upper mid zone. In advanced OA (n=20; score 18.9 ± 5.3), they were uniformly distributed in these zones and accumulated pericellularly. Perifibrillar adapter proteins are important for the stabilization of the collagen network in the upper zones of healthy cartilage. Their degradation might be a critical event in early OA. In advanced OA, there are indications for an increased synthesis in an attempt to regenerate the lost tissue and to protect the remaining cartilage from further destruction.
纤维周适配器蛋白连接胶原纤维，并将胶原网络与聚集蛋白聚糖基质相连，其似乎在骨关节炎（OA）的发病机制中起关键作用。因此，我们采用免疫组织化学方法检测了健康人（n = 4）和OA患者（n = 42）膝关节软骨样本中Ⅱ型胶原以及主要的纤维周适配器蛋白——IX型胶原、核心蛋白聚糖、软骨寡聚基质蛋白（COMP）和基质金属蛋白酶3的细胞外分布情况。使用OA评分进行组织病理学评估。根据疾病阶段评估染色模式。纤维周适配器蛋白在健康软骨的上层区域均匀分布。在中度OA（n = 8；评分14.3±4.7）中，所有分析的蛋白在纤维化区域或浅表层和上中层局部缺失。在重度OA（n = 20；评分18.9±5.3）中，它们在这些区域均匀分布并在细胞周围积聚。纤维周适配器蛋白对健康软骨上层区域胶原网络的稳定很重要。它们的降解可能是早期OA中的关键事件。在重度OA中，有迹象表明其合成增加，试图再生丢失的组织并保护剩余软骨免受进一步破坏。
