绵羊垂体前叶中的免疫反应性强啡肽 -A 及 17β-雌二醇给药的影响

Immunoreactive dynorphin-A in ovine anterior pituitary and effects of estradiol-17 beta administration.

作者信息

Chao C C, Trout W E, Malven P V

出版信息

Peptides. 1987 Mar-Apr;8(2):367-9. doi: 10.1016/0196-9781(87)90113-6.

Abstract

Anterior lobe (AL) tissue of the ovine pituitary gland contained a form of immunoreactive dynorphin-A (ir-DYN-A) larger than that found in pituitary neurointermediate lobe. Administration of estradiol-17 beta or estradiol-17 beta plus progesterone to ovariectomized sheep decreased AL tissue concentrations of ir-DYN-A but did not affect any LH parameter. Enzymatically dispersed AL cells also contained ir-DYN-A, but specific release during in vitro incubation was too low to be detected even when cells were exposed to gonadotropin-releasing hormone.

摘要

绵羊脑垂体前叶（AL）组织中含有的一种免疫反应性强啡肽A（ir-DYN-A）形式，比垂体神经中间叶中的更大。给去卵巢绵羊注射17β-雌二醇或17β-雌二醇加孕酮，可降低AL组织中ir-DYN-A的浓度，但不影响任何促黄体生成素参数。酶解分散的AL细胞也含有ir-DYN-A，但即使细胞暴露于促性腺激素释放激素，体外孵育期间的特异性释放也很低，无法检测到。