Knepel W, Schwaninger M
Naunyn Schmiedebergs Arch Pharmacol. 1985 Mar;329(1):94-6. doi: 10.1007/BF00695198.
Rat anterior pituitary quarters were incubated in vitro and the release of dynorphin A1-13-like immunoreactivity (Dyn A1-13-IR) into the incubation medium was studied. An elevation of the potassium concentration of the incubation medium to 40 mmol/l increased the release of Dyn A1-13-IR in a calcium-dependent manner. Addition of luteinizing hormone-releasing hormone (LHRH) stimulated the release of Dyn A1-13-IR in a dose-dependent manner. The LHRH-induced release of Dyn A1-13-IR was almost completely prevented by omission of calcium ions from the incubation medium or by addition of the LHRH-antagonist D-pGlu1,D-Phe2,D-Trp3,6-LHRH. This is the first demonstration that dynorphin-like immunoreactivity can be released from the rat adenohypophysis through calcium-dependent mechanisms by both high potassium concentration and receptor-mediated stimulation.
将大鼠垂体前叶切成小块进行体外培养，研究强啡肽A1 - 13样免疫反应性物质（Dyn A1 - 13 - IR）释放到培养液中的情况。将培养液中钾离子浓度提高到40 mmol/L，以钙依赖的方式增加了Dyn A1 - 13 - IR的释放。添加促黄体生成素释放激素（LHRH）以剂量依赖的方式刺激了Dyn A1 - 13 - IR的释放。培养液中钙离子缺失或添加LHRH拮抗剂D - pGlu1，D - Phe2，D - Trp3，6 - LHRH几乎完全抑制了LHRH诱导的Dyn A1 - 13 - IR释放。这首次证明了强啡肽样免疫反应性物质可通过高钾浓度和受体介导的刺激，经钙依赖机制从大鼠腺垂体释放。
