National Key Discipline, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin 150086, China.
Nutrients. 2017 Aug 26;9(9):935. doi: 10.3390/nu9090935.
The effects of maternal prenatal folic acid supplementation (FAS) on offspring lipid metabolism in adulthood remains unclear, although prenatal FAS is compulsively suggested in many countries. Female Sprague-Dawley rats were fed with control (CON) or FAS diets before and during pregnancy. Male offspring of CON and FAS dams were further divided into two groups at seven weeks for CON and high-fat (HF) diet interventions for eight weeks in adulthood ( = 10). The interactive effects of maternal prenatal FAS and offspring HF in adulthood on lipid metabolism and DNA methylation of genes involved in lipids metabolism were assessed. The male offspring of FAS dams had elevated serum and liver triglyceride level when fed with HF compared to the male offspring of CON dams. The mRNA and protein expression levels of hepatic and adipose were significantly decreased in offspring of FAS dams than in offspring of CON dams. Furthermore, maternal prenatal FAS resulted in elevated DNA methylation levels in the promoter and first exon region of hepatic and adipose in offspring. Maternal FAS exacerbated the adverse effects of HF on lipid metabolism in offspring through inducing aberrant DNA methylation levels of hepatic and adipose .
母体产前叶酸补充（FAS）对后代成年期脂质代谢的影响尚不清楚，尽管在许多国家都强制建议进行产前 FAS。雌性 Sprague-Dawley 大鼠在怀孕前和怀孕期间分别喂食对照（CON）或 FAS 饮食。CON 和 FAS 组母鼠的雄性后代在 7 周时进一步分为两组，成年期（= 10）进行 CON 和高脂肪（HF）饮食干预 8 周。评估母体产前 FAS 和成年后代 HF 对脂质代谢和参与脂质代谢的基因的 DNA 甲基化的相互作用影响。与 CON 组母鼠的雄性后代相比，FAS 组母鼠的雄性后代在喂食 HF 时血清和肝脏甘油三酯水平升高。FAS 组母鼠的后代肝脏和脂肪组织中 和 的 mRNA 和蛋白表达水平明显低于 CON 组母鼠的后代。此外，母体产前 FAS 导致后代肝脏和脂肪组织中 和 的启动子和第一外显子区域的 DNA 甲基化水平升高。母体 FAS 通过诱导肝脏和脂肪组织中 和 的异常 DNA 甲基化水平，加剧了 HF 对后代脂质代谢的不利影响。
