Moreira Anthony, Freitas Rosa, Figueira Etelvina, Volpi Ghirardini Annamaria, Soares Amadeu M V M, Radaelli Marta, Guida Marco, Libralato Giovanni
Departmento de Biologia & CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Department of Environmental Sciences, Informatics and Statistics, University Cà Foscari Venice, Via Torino 155, 30172 Venezia-Mestre, Italy.
Ecotoxicol Environ Saf. 2018 Jan;147:251-259. doi: 10.1016/j.ecoenv.2017.08.043. Epub 2017 Sep 14.
The combined effects of different salinity and temperature levels on the toxicity of Arsenic (As) were studied on the embryonic development of the oyster Crassostrea gigas. A standardized embryotoxicity test was performed to assess the interactive effects of these stressors, in a full factorial design experiment including a range of salinities (15, 19, 24, 28 and 32), temperatures (16, 20, 24, 28 and 32°C) and As concentrations (100, 300, 600, 1200, 2400µgL). The embryotoxicity endpoint was about the determination of normal larvae development rates at various conditions, and median effect concentration (EC) determination for each As exposure condition. Results showed that toxicity induced by As was characterized by retardation of embryonic development observing toxic effects at lower concentrations than previously reported studies. The presence of As in seawater resulted in a narrower range of tolerance to both salinity and temperature. These findings bring new insights on the impacts of a common contaminant on an important shellfish species having a planktonic early life stage development, with potential implications for population survival and ecosystem functioning in a changing environment.
研究了不同盐度和温度水平对砷（As）毒性的综合影响，对象是太平洋牡蛎（Crassostrea gigas）的胚胎发育。进行了标准化的胚胎毒性试验，以评估这些应激源的交互作用，试验采用全因子设计，涵盖一系列盐度（15、19、24、28和32）、温度（16、20、24、28和32°C）以及砷浓度（100、300、600、1200、2400µgL）。胚胎毒性终点是确定不同条件下正常幼虫的发育速率，以及每种砷暴露条件下的半数效应浓度（EC）。结果表明，砷诱导的毒性表现为胚胎发育迟缓，在比先前报道的研究更低的浓度下即可观察到毒性效应。海水中存在砷导致对盐度和温度的耐受范围变窄。这些发现为一种常见污染物对具有浮游幼体早期生活阶段发育的重要贝类物种的影响带来了新的见解，对不断变化的环境中的种群生存和生态系统功能具有潜在影响。
