Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 15;189:510-515. doi: 10.1016/j.saa.2017.08.056. Epub 2017 Aug 24.
In this work, we developed a colorimetric biosensing system for glucose detection by coupling the peroxidase-like of ficin and the glucose oxidase (GOx). GOx can catalyze the oxidation of glucose to produce HO, then, ficin catalyzes the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) by HO to produce a blue color reaction. The present sensing system showed a linear response toward glucose detection over range of 2.0-100μM with a detection limit of 0.5μM. This system is simple, low cost, highly sensitive and selective for glucose detection, and was also applied to measuring glucose in human serum. Furthermore, in order to expand the application of ficin in biological sensing, we immobilized ficin onto the SiO@FeO NPs, which exhibited the merits of recycling as well as allowing the repeated detection of glucose. Thus it may provide great potential applications in biomedicine, biotechnology and environmental chemistry.
在这项工作中，我们通过将木瓜蛋白酶的过氧化物酶样活性与葡萄糖氧化酶（GOx）偶联，开发了一种用于葡萄糖检测的比色生物传感系统。GOx 可以催化葡萄糖氧化生成 HO ，然后，木瓜蛋白酶通过 HO 催化过氧化物酶底物 3,3',5,5'-四甲基联苯胺（TMB）的氧化产生蓝色反应。该传感系统在 2.0-100μM 的葡萄糖检测范围内呈现线性响应，检测限为 0.5μM。该系统具有简单、低成本、高灵敏度和选择性，可用于检测人血清中的葡萄糖。此外，为了扩展木瓜蛋白酶在生物传感中的应用，我们将其固定在 SiO@FeO NPs 上，该 NPs 具有可回收和允许重复检测葡萄糖的优点。因此，它可能在生物医学、生物技术和环境化学领域具有巨大的潜在应用价值。
