School of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China; Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA.
School of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, China.
Bioresour Technol. 2017 Nov;244(Pt 1):957-962. doi: 10.1016/j.biortech.2017.08.024. Epub 2017 Aug 8.
Broussonetia papyrifera, known as paper mulberry, is a potential feed stock for bioethanol production because of its cellulose-rich composition. Lignin in dilute acid pretreated Broussonetia papyrifera was fractionated to three different fractions, and their physiochemical properties were determined by FT-IR, GPC and NMR analyses. Different structural characteristics were observed from each lignin fraction. Cellobiohydrolases I (CBH) adsorption to each lignin was understood by the lignin properties. The results showed that aliphatic hydroxyl groups in lignin showed positive correlations with the maximum binding ability of CBH onto lignin samples. Also, the contents of phenolic compounds such as p-hydroxyphenyl benzoate (PB), syringyl (S) and guaiacyl (G) units in the lignin influenced their CBH binding.
构树，又称纸桑，由于其富含纤维素的组成，是生物乙醇生产的潜在原料。用稀酸预处理构树得到的木质素被分离成三个不同的部分，并通过傅里叶变换红外光谱（FT-IR）、凝胶渗透色谱（GPC）和核磁共振（NMR）分析来确定它们的理化性质。从每个木质素部分观察到不同的结构特征。通过木质素的性质来理解纤维素酶 I（CBH）对每个木质素的吸附。结果表明，木质素中的脂肪羟基与 CBH 对木质素样品的最大结合能力呈正相关。此外，木质素中对羟苯基苯甲酸酯（PB）、愈创木基（S）和松柏基（G）单元等酚类化合物的含量也影响其 CBH 的结合。
