Sakurai J, Nagahama M
Toxicon. 1987;25(3):279-84. doi: 10.1016/0041-0101(87)90256-x.
When one residue of tyrosine per molecule of epsilon toxin was nitrated by tetranitromethane, the modified toxin lost more than 90% of its original activity. The toxin also was inactivated by treatment with N-acetylimidazole. The lethality was restored when the inactive N-acetylimidazole-treated toxin was treated with hydroxylamine. The inactive N-acetylimidazole-treated toxin was not nitrated by tetranitromethane under conditions where one residue of Tyr per molecule of the untreated toxin is nitrated. These data suggest that only one Tyr residue in the toxin is important for the lethal activity.
当用四硝基甲烷将ε毒素分子中的一个酪氨酸残基硝化时，修饰后的毒素失去了其原有活性的90%以上。该毒素也可通过用N - 乙酰咪唑处理而失活。当用羟胺处理无活性的经N - 乙酰咪唑处理的毒素时，其致死性得以恢复。在未处理的毒素分子中每分子有一个酪氨酸残基被硝化的条件下，无活性的经N - 乙酰咪唑处理的毒素不会被四硝基甲烷硝化。这些数据表明，毒素中只有一个酪氨酸残基对致死活性很重要。
