Nagahama M, Takahashi T, Sakurai J
Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
FEMS Microbiol Lett. 1990 Oct;60(1-2):59-62. doi: 10.1016/0378-1097(90)90345-q.
Lethal and pressor activities, and the contractile responses of rat isolated ileum to Clostridium perfringens epsilon toxin, were significantly prevented by the prior administration of epsilon toxin inactivated by 1-ethyl-3-(3-diethyl-aminopropyl) carbodiimide in the presence of glycine methyl ester (EDC), 2,4,6-trinitrobenzene sulfonic acid (TNBS), succinic anhydride (SA) and ethoxyformic anhydride (EFA). However, the prior administration of the toxin inactivated by N-acetylimidazole (NAI), tetranitromethane (TNM) and N-bromosuccinimide (NBS) resulted in no inhibition of these biological activities. These data suggest that the toxin interacts with specific site(s) on target organs or tissues. The relationship between amino acid residues and the actions of the toxin is described.
致死活性和升压活性，以及大鼠离体回肠对产气荚膜梭菌ε毒素的收缩反应，在甘氨酸甲酯（EDC）、2,4,6-三硝基苯磺酸（TNBS）、琥珀酸酐（SA）和乙氧基甲酸酐（EFA）存在的情况下，预先给予经1-乙基-3-(3-二乙氨基丙基)碳二亚胺灭活的ε毒素可显著抑制。然而，预先给予经N-乙酰咪唑（NAI）、四硝基甲烷（TNM）和N-溴代琥珀酰亚胺（NBS）灭活的毒素对这些生物活性没有抑制作用。这些数据表明该毒素与靶器官或组织上的特定位点相互作用。描述了氨基酸残基与毒素作用之间的关系。
