使用环介导等温扩增法快速视觉检测百合斑驳病毒
Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method.
作者信息
Zhao Binan, Yang Dan, Zhang Yongchun, Xu Yan, Zhao Xiao, Liang Jieling, Fan Xiaorui, Du Yanan, Zhu Ziping, Shi Bin, Zhang Qi, Zhang Xiaoxia, Cai Youming, Zhao Kai
机构信息
Department of Clinical Medicine, XuZhou Medical University, Xuzhou, People's Republic of China.
School of Life science, Taizhou University, Taizhou, People's Republic of China.
出版信息
Arch Virol. 2018 Feb;163(2):545-548. doi: 10.1007/s00705-017-3618-4. Epub 2017 Nov 14.
Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.
百合斑驳病毒（LMoV；马铃薯Y病毒属，马铃薯Y病毒科）感染百合属植物，导致花朵和种球质量下降。开发了一种快速灵敏的环介导等温扩增（LAMP）检测方法来检测LMoV的外壳蛋白基因。这种LAMP方法对LMoV具有高度特异性，与其他百合病毒无交叉反应。使用LAMP检测法检测LMoV的灵敏度比使用传统聚合酶链反应高100倍。随后成功应用逆转录LAMP（RT-LAMP）检测LMoV RNA。新建立的LAMP和一步法RT-LAMP为检测百合植株中的LMoV提供了一种替代方法。
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