Sow Doudou, Dieng Yémou, Haouchine Djamal, Niang Khadim, Niang Thiane, Sylla Khadime, Tine Roger Clément, Ndiaye Magatte, Ndiaye Jean Louis, Faye Babacar, Faye Omar, Gaye Oumar, Dieng Thérèse, Izri Arezki
Service de Parasitologie-Mycologie, faculté de médecine, Université Cheikh Anta Diop, BP 5005-Fann, Dakar, Sénégal.
CHU Avicenne, Université Paris 13125, rue de Stalingrad, 93009 Bobigny Cedex, France.
J Parasit Dis. 2017 Sep;41(3):814-822. doi: 10.1007/s12639-017-0894-6. Epub 2017 Feb 28.
In the context of controlling intestinal parasites, accurate diagnosis is essential. Our objective was to evaluate the performance of new diagnostic kits compared to conventional microscopic methods in identifying intestinal parasites. Faeces collected in rural area in Senegal were subjected to several detection techniques. Thus, the sensitivity, specificity, positive and negative predictive values of new diagnostic techniques were compared to conventional merthiolate-iodine-formalin, conventional Bailenger and modified Ritchie. Furthermore, the kappa coefficient was calculated to evaluate the correlation between the new kit and those of modified Ritchie. Out of the 117 patients examined, 102 presented with a parasite, or prevalence of 87.1%. The Fumouze techniques proved to be as effective as the conventional methods in detecting flagellates and helminths with sensitivities ranging from 97 to 100%. However, conventional techniques were slightly more sensitive in identifying and . The correlation was nearly perfect (k = 0.83 and 1), respectively between Bailenger Fumouze, Iodesine Fumouze and modified Ritchie in identifying helminths while it was just acceptable (k = 0.27 and 0.28) in identifying . The modified Ritchie technique routinely used in our laboratory remains a good diagnostic tool. However, the use of kit techniques was interesting when reading the pellet after concentration and the Colour KOP staining was a considerable contribution to the diagnosis of the vegetative forms. Therefore, it would be interesting to determine the cost of a stool test using Fumouze kit techniques to provide the most cost effective way.
在控制肠道寄生虫的背景下,准确诊断至关重要。我们的目标是评估新型诊断试剂盒与传统显微镜检查方法在识别肠道寄生虫方面的性能。在塞内加尔农村地区收集的粪便样本接受了多种检测技术。因此,将新型诊断技术的敏感性、特异性、阳性和阴性预测值与传统的硫柳汞 - 碘 - 福尔马林法、传统的贝伦热法和改良的里奇法进行了比较。此外,计算了kappa系数以评估新型试剂盒与改良里奇法之间的相关性。在接受检查的117名患者中,有102名感染了寄生虫,患病率为87.1%。富穆兹技术在检测鞭毛虫和蠕虫方面被证明与传统方法一样有效,敏感性范围为97%至100%。然而,传统技术在识别[此处原文缺失部分内容]时略为敏感。在识别蠕虫方面,贝伦热富穆兹法、碘色富穆兹法与改良里奇法之间的相关性几乎完美(kappa值分别为0.83和1),而在识别[此处原文缺失部分内容]时相关性仅为可接受水平(kappa值分别为0.27和0.28)。我们实验室常规使用的改良里奇技术仍然是一种很好的诊断工具。然而,在浓缩后读取沉淀时使用试剂盒技术很有意义,并且彩色KOP染色对营养体形式的诊断有很大贡献。因此,确定使用富穆兹试剂盒技术进行粪便检测的成本以提供最具成本效益的方法将是很有意义的。