Autier Brice, Gangneux Jean-Pierre, Robert-Gangneux Florence
Univ Rennes, CHU Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé Environnement Travail), UMR_S 1085, 35000 Rennes, France.
Microorganisms. 2022 Jun 16;10(6):1237. doi: 10.3390/microorganisms10061237.
The diagnosis of intestinal parasitic infections still widely relies on microscopic examination of stools and requires reliable reagents and staff expertise. The ParaFlo® assays (Eurobio Ingen) are ready-to-use concentration methods for parasite egg detection, and they could improve reagent traceability and ease of manipulation. Ninety-three stool samples were analyzed with the ParaFlo® concentration methods and then compared with routine microscopic methods for protozoa and helminth detection: seventy-eight were analyzed with ParaFlo® Bailenger and in-house Thebault or Bailenger concentrations, and fifty-five were analyzed with ParaFlo®DC and the in-house merthiolate-formalin diphasic concentration (DC) method. Fully concordant results were obtained for 75%, 70%, and 69% of samples when comparing ParaFlo® DC and in-house DC, ParaFlo® Bailenger and in-house Bailenger, and ParaFlo® Bailenger and Thebault, respectively. The performances of the ParaFlo® assays did not differ statistically from that obtained with their in-house counterparts (Bailenger and DC) for the detection of protozoa, but ParaFlo® Bailenger performed significantly poorer than the Thebault method (p < 0.001). No statistical differences were observed between the commercial and in-house methods for helminth detection. These marketed concentration methods could be used in routine if combined with other techniques for protozoa detection.
肠道寄生虫感染的诊断目前仍广泛依赖粪便显微镜检查，且需要可靠的试剂和专业的工作人员。ParaFlo®检测法（欧洲生物公司）是用于检测寄生虫卵的即用型浓缩方法，可提高试剂的可追溯性并便于操作。采用ParaFlo®浓缩方法对93份粪便样本进行分析，然后与用于原生动物和蠕虫检测的常规显微镜方法进行比较：78份样本采用ParaFlo®贝伦热法以及内部的泰博尔特法或贝伦热法进行浓缩分析，55份样本采用ParaFlo®DC法和内部的硫柳汞 - 福尔马林双相浓缩（DC）法进行分析。在比较ParaFlo®DC法和内部DC法、ParaFlo®贝伦热法和内部贝伦热法、ParaFlo®贝伦热法和泰博尔特法时，分别有75%、70%和69%的样本获得了完全一致的结果。对于原生动物检测，ParaFlo®检测法的性能与相应的内部方法（贝伦热法和DC法）相比，在统计学上没有差异，但ParaFlo®贝伦热法的表现明显逊于泰博尔特法（p < 0.001）。在蠕虫检测方面，商业方法和内部方法之间未观察到统计学差异。如果与其他原生动物检测技术相结合，这些市售的浓缩方法可用于常规检测。
