Dobiesz Malwina, Piotrowicz-Cieślak Agnieszka I
Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynOlsztyn, Poland.
Front Plant Sci. 2017 Aug 11;8:1392. doi: 10.3389/fpls.2017.01392. eCollection 2017.
The aim of the study was to evaluate the vigor and viability as well as to determine and compare the contents of selected protein fractions of white lupin ( L.) seeds stored for 26 years at temperatures of -14°C and +20°C. The seeds stored at -14°C germinated in 86.3%, while the seeds stored at +20°C did not germinate at all. The viability evaluation was confirmed by the measuring electroconductivity of seed exudates. In seeds stored at -14°C the contents of γ, δ, and β conglutin were 14, 4 and 69 mg g fresh mass, respectively, while in seed stored at +20°C they were 15.5, 3, 65 mg g fresh mass, respectively. One-dimensional electrophoresis of γ and δ conglutin fractions indicated the presence of several intense polypeptide bands with molecular weights from 23.0 to 10.3 kDa. Polypeptide bands with a molecular weight of 22.4 and 19.8 kDa exhibited almost two times higher expression in the seeds stored at -14°C compared to the seeds stored at +20°C. Electrophoresis revealed 310 protein spots on the maps generated for seeds stored at -14°C, and 228 spots for seeds stored at +20°C. In seeds stored at +20°C most polypeptide subunits had a pI ranging from 4.5 to 7 and a molecular weight of 10-97 kDa. The greatest differences in the contents of polypeptides between the analyzed variants was observed within the range of 20-45 kDa (-14°C: 175, +20°C: 115 protein spots) and within the range of 65-97 kDa (-14°C: 103, +20°C: 75 protein spots). In seeds stored at +20°C, a clear decline in basic (8-10 pI) polypeptides was observed. The study demonstrated that the polypeptides identified as γ and δ conglutins are probably closely related to vigor and viability of seeds.
本研究的目的是评估白羽扇豆（L.）种子在-14°C和+20°C温度下储存26年后的活力和生存力，并测定和比较所选蛋白质组分的含量。储存在-14°C的种子发芽率为86.3%，而储存在+20°C的种子根本没有发芽。通过测量种子渗出液的电导率来确认活力评估。储存在-14°C的种子中，γ、δ和β伴刀豆球蛋白的含量分别为14、4和69毫克/克鲜重，而储存在+20°C的种子中，它们分别为15.5、3、65毫克/克鲜重。γ和δ伴刀豆球蛋白组分的一维电泳表明存在几条强多肽带，分子量在23.0至10.3 kDa之间。分子量为22.4和19.8 kDa的多肽带在储存在-14°C的种子中的表达比储存在+20°C的种子中高出近两倍。电泳显示，储存在-14°C的种子图谱上有310个蛋白质斑点，储存在+20°C的种子图谱上有228个斑点。储存在+20°C的种子中，大多数多肽亚基的pI范围为4.5至7，分子量为10-97 kDa。在所分析的变体之间，多肽含量的最大差异出现在20-45 kDa范围内（-14°C：175个，+20°C：115个蛋白质斑点）和65-97 kDa范围内（-14°C：103个，+20°C：75个蛋白质斑点）。在储存在+20°C的种子中，观察到碱性（8-10 pI）多肽明显减少。该研究表明，鉴定为γ和δ伴刀豆球蛋白的多肽可能与种子的活力和生存力密切相关。
