Department of Orthopedics, Xiangyang No. 1 People's Hospital, Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China.
Department of Nephrology, Xiangyang No. 1 People's Hospital, Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China.
Mol Med Rep. 2017 Oct;16(4):5568-5574. doi: 10.3892/mmr.2017.7256. Epub 2017 Aug 16.
Osteosarcoma (OS) is the most common primary bone cancer, and frequently leads to mortality as a result of rapid proliferation and metastasis. Recent data highlight the important role of microRNAs (miRs) in human cancers, including OS, providing a novel method for its diagnosis and treatment. In the present study, it was identified that miR‑491‑3p expression was frequently decreased in OS tissues and OS cell lines. Restored miR‑491‑3p expression suppressed the growth and invasion of OS cells. Bioinformatics and experimental analysis indicated that tetraspanin 1 (TSPAN1) is a direct target of miR‑491‑3p in OS. The TSPAN1 mRNA level was inversely associated with the miR‑491‑3p level in OS tissues and cell lines. Consistent with this, TSPAN1 knockdown inhibited the growth and invasion of OS cells. In conclusion, these data provide compelling evidence that miR‑491‑3p functions as a tumor suppressor in OS to attenuate the potential of growth and invasion by targeting TSPAN1.
骨肉瘤（OS）是最常见的原发性骨癌，由于其快速增殖和转移，常导致患者死亡。最近的数据强调了 microRNAs（miRs）在包括 OS 在内的人类癌症中的重要作用，为其诊断和治疗提供了一种新方法。在本研究中，发现 miR-491-3p 在 OS 组织和 OS 细胞系中表达经常下调。恢复 miR-491-3p 的表达抑制了 OS 细胞的生长和侵袭。生物信息学和实验分析表明，四跨膜蛋白 1（TSPAN1）是 OS 中 miR-491-3p 的直接靶标。OS 组织和细胞系中 TSPAN1 mRNA 水平与 miR-491-3p 水平呈负相关。与此一致，TSPAN1 敲低抑制了 OS 细胞的生长和侵袭。总之，这些数据提供了令人信服的证据，表明 miR-491-3p 在 OS 中作为肿瘤抑制因子发挥作用，通过靶向 TSPAN1 减弱生长和侵袭的潜力。
